白细胞介素-12B前体和谷胱甘肽S-转移酶P1基因多态性与矽肺的关系

IL-12Bpro and GSTP1 polymorphisms in association with silicosis.

作者信息

Stanilova S, Miteva L, Prakova G

机构信息

Department of Molecular Biology, Immunology & Medical Genetics, Faculty of Medicine, Trakia University, Stara Zagora, Bulgaria.

出版信息

Tissue Antigens. 2008 Feb;71(2):169-74. doi: 10.1111/j.1399-0039.2007.00985.x. Epub 2007 Dec 13.

DOI:10.1111/j.1399-0039.2007.00985.x

PMID:18086266

Abstract

To assess the role of IL-12Bpro and GSTP1 polymorphisms on induced interleukin (IL)-12p40 production in respect to silicosis development, we examined their distribution in 63 silicosis patients and 165 healthy donors. Decreased frequencies of IL-12Bpro-2 allele (42% vs 62.5%, P = 0.0042) and genotype 22 (19% vs 40%, P = 0.02) in patients compared with exposed healthy donors suggested their protective role in silicosis susceptibility. The GSTP1 A/G polymorphism was also associated with silicosis susceptibility. The allele G and genotype GG were overrepresented among patients than among healthy men (36% vs 23%, P = 0.013; 14% vs 2%, P(c) = 0.012). Also, patients' peripheral blood mononuclear cell (PBMC) produced higher IL-12p40 than healthy donors' depending on both IL-12Bpro and GSTP1 polymorphisms. In conclusion, homozygosity of high producer IL-12p40 genotype IL-12Bpro-11 and GSTP1-GG had the highest genetic risk of silicosis development in Bulgarian miners.

摘要

为评估白细胞介素（IL）-12Bpro和谷胱甘肽S-转移酶P1（GSTP1）基因多态性在矽肺发生过程中对诱导产生IL-12p40的作用，我们检测了63例矽肺患者和165名健康供者中这些基因多态性的分布情况。与暴露的健康供者相比，患者中IL-12Bpro -2等位基因频率降低（42%对62.5%，P = 0.0042），基因型22频率降低（19%对40%，P = 0.02），提示它们在矽肺易感性中起保护作用。GSTP1 A/G基因多态性也与矽肺易感性相关。患者中G等位基因和GG基因型的比例高于健康男性（36%对23%，P = 0.013；14%对2%，P(c) = 0.012）。此外，根据IL-12Bpro和GSTP1基因多态性，患者外周血单个核细胞（PBMC）产生的IL-12p40高于健康供者。总之，在保加利亚矿工中，高产生者IL-12p40基因型IL-12Bpro -11和GSTP1 - GG的纯合子具有最高的矽肺发生遗传风险。