聚（rC）结合蛋白2与柯萨奇病毒B3的寡聚（rC）序列相互作用。

Poly(rC)-binding protein 2 interacts with the oligo(rC) tract of coxsackievirus B3.

作者信息

Zell Roland, Ihle Yvonne, Seitz Simone, Gündel Ulrike, Wutzler Peter, Görlach Matthias

机构信息

Institute of Virology and Antiviral Therapy, Friedrich Schiller University, Hans-Knoell-Strasse 2, D-07745 Jena, Germany.

出版信息

Biochem Biophys Res Commun. 2008 Feb 22;366(4):917-21. doi: 10.1016/j.bbrc.2007.12.038. Epub 2007 Dec 18.

DOI:10.1016/j.bbrc.2007.12.038

PMID:18086560

Abstract

The human poly(rC)-binding protein (PCBP) 2 is known to interact with enteroviral RNA. Here, the interaction of PCBP2 with RNA target sequences at the 5' end of the coxsackievirus B3 genome was investigated. Using the electrophoretic mobility shift assay and the yeast three-hybrid system, a short oligo(rC) tract connecting cloverleaf and IRES is demonstrated to contribute to PCBP2 binding. This oligo(rC) tract is conserved among entero- and rhinoviruses. In absence of the viral 3C proteinase, an extended cloverleaf RNA (nt 1-105) containing the oligo(rC) tract interacts with PCBP2 whereas the cloverleaf (nt 1-87) lacking the oligo(rC) tract does not. In the presence of 3C proteinase, cloverleaf RNA (1-87) interacts with PCBP2.

摘要

已知人类多聚（rC）结合蛋白（PCBP）2可与肠道病毒RNA相互作用。在此，研究了PCBP2与柯萨奇病毒B3基因组5'端RNA靶序列的相互作用。使用电泳迁移率变动分析和酵母三杂交系统，证明连接三叶形结构和内部核糖体进入位点（IRES）的一段短寡聚（rC）序列有助于PCBP2结合。这段寡聚（rC）序列在肠道病毒和鼻病毒中保守。在没有病毒3C蛋白酶的情况下，包含该寡聚（rC）序列的延伸三叶形RNA（第1至105位核苷酸）与PCBP2相互作用，而缺少该寡聚（rC）序列的三叶形结构（第1至87位核苷酸）则不与PCBP2相互作用。在有3C蛋白酶存在的情况下，三叶形RNA（1至87）与PCBP2相互作用。