使用串联结合位点增强DNA足迹
DNA footprint enhancement using tandem binding sites.
作者信息
Zhang W W, Farrés J, Busch H
机构信息
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
出版信息
Biotechniques. 1991 Dec;11(6):728, 730, 732-3.
PMID:1809324
Abstract
A concatenated DNA fragment containing a five-repeat binding site was used for DNase I footprinting. Under the same conditions, the tandem repeat assay greatly enhanced the DNA footprint as compared with a native DNA sequence with only one binding site. This technique provides an approach for improving poor DNA footprints.
摘要
一个包含五个重复结合位点的串联DNA片段被用于DNase I足迹分析。在相同条件下，与仅具有一个结合位点的天然DNA序列相比，串联重复分析极大地增强了DNA足迹。该技术为改善不佳的DNA足迹提供了一种方法。
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