Park Su Yon, Kim Meoung Hee, Kang So Young, Suh Jin Tae, Lee Woo In
Department of Laboratory Medicine, Kyunghee Medical Center, Kyunghee University, College of Medicine, Seoul, Korea.
Korean J Lab Med. 2007 Jun;27(3):197-204. doi: 10.3343/kjlm.2007.27.3.197.
Ischemic stroke is a complex condition influenced by many factors. Previous studies have demonstrated that inflammatory markers might play a role in such vascular diseases. Therefore the purpose of this study was to compare the expression of inflammatory markers in Korean ischemic stroke patients and to investigate their relationship to APOE polymorphism.
The patient group consisted of 275 patients with large artery atherosclerosis (LAA, n=169) and small artery occlusion (SAO, n=106). One hundred and nineteen age matched healthy subjects were recruited as the control group. Serum levels of three inflammatory markers (matrix metalloproteinase, MMP-9; tissue inhibitor of metalloproteinase-1, TIMP-1; and high-sensitivity C-reactive protein, hs-CRP) were measured in each patient by using commercially available kits. Comparison of clinical risk factors, inflammatory marker levels, and APOE genotypes between the stroke patient group and control group and between the two patient subgroups was assessed.
Comparison of the stroke group to control group showed significantly elevated levels of circulating MMP-9 (P<0.01) and hs-CRP (P=0.01). Comparison between the individual subgroups revealed a significantly higher level of only TIMP-1 in the LAA subgroup compared to the SAO subgroup (P<0.01). There was no significant difference in inflammatory marker levels among each allele carrier.
The present study revealed the obvious tendency of increased circulating inflammatory markers in the patients with acute ischemic attack, especially MMP-9 and hs-CRP. Our observations suggest that measurement of serum MMP-9, TIMP-1, and hs-CRP levels may be useful in the diagnosis of ischemic stroke patients.
缺血性中风是一种受多种因素影响的复杂病症。先前的研究表明，炎症标志物可能在这类血管疾病中发挥作用。因此，本研究的目的是比较韩国缺血性中风患者炎症标志物的表达情况，并研究它们与载脂蛋白E（APOE）基因多态性的关系。
患者组由275例患者组成，其中大动脉粥样硬化（LAA，n = 169）和小动脉闭塞（SAO，n = 106）。招募119名年龄匹配的健康受试者作为对照组。使用市售试剂盒测量每位患者血清中的三种炎症标志物（基质金属蛋白酶，MMP-9；金属蛋白酶组织抑制剂-1，TIMP-1；和高敏C反应蛋白，hs-CRP）水平。评估中风患者组与对照组以及两个患者亚组之间临床危险因素、炎症标志物水平和APOE基因型的比较。
中风组与对照组比较显示，循环中的MMP-9（P<0.01）和hs-CRP（P = 0.01）水平显著升高。各亚组之间的比较显示，与SAO亚组相比，LAA亚组中仅TIMP-1水平显著更高（P<0.01）。各等位基因携带者之间的炎症标志物水平无显著差异。
本研究揭示了急性缺血性发作患者循环炎症标志物升高的明显趋势，尤其是MMP-9和hs-CRP。我们的观察结果表明，测量血清MMP-9、TIMP-1和hs-CRP水平可能有助于缺血性中风患者的诊断。
