[高效液相色谱法检测甲基苯丙胺和苯丙胺]

[Detecting methamphetamine and amphetamine with high performance liquid chromatography].

作者信息

Fu Qiang, Liao Lin-chuan, Chen Li-li, Yan You-yi, Yang Lin, Hou Jun-hong, Chen Yu

机构信息

Department of Forensic Toxicological Analysis, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Nov;38(6):1025-8.

PMID:18095613

Abstract

OBJECTIVE

To establish a method for detecting methamphetamine (MA) and amphetamine (AMP) with high performance liquid chromatography (HPLC).

METHODS

Both MA and AMP were isolated on a C18 column and methanol-phosphate buffer (0.015 mol/L NaH2PO4) at a flow rate of 1.0 mL/min. The 190-360 nm ultraviolet spectrum was examined, with 215 nm as the detection wavelength.

RESULTS

The MA and AMP were well isolated and determined. The MA determined by the HPLC had good linearity with the real value at the range from 1.4 to 270 microg/mL (R2=1), with an average recovery rate of 102.5%. The detectable Limit was 0.73 microg/mL (S/N > or =3). The AMP determined by the HPLC had a good linearity with the real value at the range from 0.9 to 580 microg/mL (R2 = 0.9999), with an average recovery rate of 101.7%. The detectable limit was 0.52 microg/mL (S/N > or =3). Both intra-day and inter-day precisions expressed by relative standard deviations of the MA and AMP were less than 2.4%.

CONCLUSION

This is a simple, rapid and accurate method for detecting methamphetamine and amphetamine.

摘要

目的

建立一种用高效液相色谱法（HPLC）检测甲基苯丙胺（MA）和苯丙胺（AMP）的方法。

方法

MA和AMP均在C18柱上，以甲醇 - 磷酸盐缓冲液（0.015 mol/L NaH2PO4）分离，流速为1.0 mL/min。检测190 - 360 nm紫外光谱，以215 nm作为检测波长。

结果

MA和AMP得到良好分离和测定。HPLC测定的MA在1.4至270μg/mL范围内与实际值具有良好线性（R2 = 1），平均回收率为102.5%。检测限为0.73μg/mL（S/N≥3）。HPLC测定的AMP在0.9至580μg/mL范围内与实际值具有良好线性（R2 = 0.9999），平均回收率为101.7%。检测限为0.52μg/mL（S/N≥3）。MA和AMP日内和日间精密度以相对标准偏差表示均小于2.4%。