• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Overproduction, purification, crystallization and preliminary X-ray analysis of the peroxiredoxin domain of a larger natural hybrid protein from Thermotoga maritima.来自嗜热栖热菌的一种较大天然杂交蛋白的过氧化物还原酶结构域的过量生产、纯化、结晶及初步X射线分析。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):29-31. doi: 10.1107/S1744309107064391. Epub 2007 Dec 20.
2
Crystallization and preliminary X-ray crystallographic analysis of the surE protein from Thermotoga maritima.嗜热栖热菌surE蛋白的结晶及初步X射线晶体学分析
Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):612-3. doi: 10.1107/s0907444901002141.
3
Crystallization and preliminary crystallographic analysis of an esterase with a novel domain from the hyperthermophile Thermotoga maritima.来自嗜热栖热菌（Thermotoga maritima）的具有新型结构域的酯酶的结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt 9):777-9. doi: 10.1107/S174430910703953X. Epub 2007 Aug 25.
4
Crystallization and preliminary X-ray crystallographic analysis of CheW from Thermotoga maritima: a coupling protein of CheA and the chemotaxis receptor.嗜热栖热菌CheW的结晶及初步X射线晶体学分析：CheA与趋化性受体的偶联蛋白
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt 4):504-6. doi: 10.1107/S1744309111005951. Epub 2011 Mar 30.
5
Overexpression, crystallization and preliminary X-ray crystallographic analysis of β-N-acetylglucosaminidase from Thermotoga maritima encoded by the Tm0809 gene.由Tm0809基因编码的嗜热栖热菌β-N-乙酰氨基葡萄糖苷酶的过表达、结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Feb 1;69(Pt 2):115-7. doi: 10.1107/S1744309112049020. Epub 2013 Jan 30.
6
Crystallization and preliminary X-ray analysis of Thermotoga maritima ribosome recycling factor.嗜热栖热菌核糖体循环因子的结晶及初步X射线分析
Acta Crystallogr D Biol Crystallogr. 1999 Dec;55(Pt 12):2049-50. doi: 10.1107/s0907444999012494.
7
Crystallization and preliminary X-ray crystallographic analysis of the TM1442 gene product from Thermotoga maritima, a homologue of Bacillus subtilis anti-anti-sigma factors.海栖热袍菌TM1442基因产物（枯草芽孢杆菌抗抗σ因子的同源物）的结晶及初步X射线晶体学分析
Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):276-8. doi: 10.1107/s0907444900016243.
8
Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals from Thermotoga maritima and Escherichia coli.来自嗜热栖热菌和大肠杆菌的八聚异戊二烯焦磷酸合酶晶体的初步X射线衍射分析。
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2265-8. doi: 10.1107/s0907444903018985. Epub 2003 Nov 27.
9
Crystallization and preliminary X-ray crystallographic analysis of Thermotoga maritima CheA P3-P4-P5 domains in complex with CheW.嗜热栖热菌CheA的P3-P4-P5结构域与CheW复合物的结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):713-5. doi: 10.1107/S174430911201826X. Epub 2012 May 24.
10
Expression, purification, crystallization and preliminary X-ray diffraction studies of bacterial and archaeal L4 ribosomal proteins.细菌和古细菌L4核糖体蛋白的表达、纯化、结晶及初步X射线衍射研究
Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):645-7. doi: 10.1107/s0907444900002638.

本文引用的文献

1
Reduction of 1-Cys peroxiredoxins by ascorbate changes the thiol-specific antioxidant paradigm, revealing another function of vitamin C.抗坏血酸对1-半胱氨酸过氧化物酶的还原作用改变了硫醇特异性抗氧化模式，揭示了维生素C的另一种功能。
Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):4886-91. doi: 10.1073/pnas.0700481104. Epub 2007 Mar 14.
2
Plant glutathione peroxidases are functional peroxiredoxins distributed in several subcellular compartments and regulated during biotic and abiotic stresses.植物谷胱甘肽过氧化物酶是功能性过氧化物酶，分布于多个亚细胞区室，并在生物和非生物胁迫期间受到调控。
Plant Physiol. 2006 Dec;142(4):1364-79. doi: 10.1104/pp.106.089458. Epub 2006 Oct 27.
3
GPX2, encoding a phospholipid hydroperoxide glutathione peroxidase homologue, codes for an atypical 2-Cys peroxiredoxin in Saccharomyces cerevisiae.GPX2编码一种磷脂氢过氧化物谷胱甘肽过氧化物酶同系物，在酿酒酵母中编码一种非典型的2-半胱氨酸过氧化物酶。
J Biol Chem. 2005 Dec 23;280(51):42078-87. doi: 10.1074/jbc.M508622200. Epub 2005 Oct 26.
4
Plant peroxiredoxins: alternative hydroperoxide scavenging enzymes.植物过氧化物酶：替代性氢过氧化物清除酶
Photosynth Res. 2002;74(3):259-68. doi: 10.1023/A:1021218932260.
5
The plant multigenic family of thiol peroxidases.植物硫醇过氧化物酶多基因家族。
Free Radic Biol Med. 2005 Jun 1;38(11):1413-21. doi: 10.1016/j.freeradbiomed.2004.07.037.
6
Poplar peroxiredoxin Q. A thioredoxin-linked chloroplast antioxidant functional in pathogen defense.杨树过氧化物还原酶Q。一种与硫氧还蛋白相关的叶绿体抗氧化剂，在病原体防御中发挥作用。
Plant Physiol. 2004 Mar;134(3):1027-38. doi: 10.1104/pp.103.035865. Epub 2004 Feb 19.
7
Structure, mechanism and regulation of peroxiredoxins.过氧化物酶的结构、机制与调控
Trends Biochem Sci. 2003 Jan;28(1):32-40. doi: 10.1016/s0968-0004(02)00003-8.
8
Peroxiredoxins.过氧化物酶
Biol Chem. 2002 Mar-Apr;383(3-4):347-64. doi: 10.1515/BC.2002.040.
9
Two GPX-like proteins from Lycopersicon esculentum and Helianthus annuus are antioxidant enzymes with phospholipid hydroperoxide glutathione peroxidase and thioredoxin peroxidase activities.来自番茄和向日葵的两种类谷胱甘肽过氧化物酶蛋白是具有磷脂氢过氧化物谷胱甘肽过氧化物酶和硫氧还蛋白过氧化物酶活性的抗氧化酶。
Eur J Biochem. 2002 May;269(9):2414-20. doi: 10.1046/j.1432-1033.2002.02905.x.
10
Glutaredoxin-dependent peroxiredoxin from poplar: protein-protein interaction and catalytic mechanism.来自杨树的谷氧还蛋白依赖性过氧化物酶：蛋白质-蛋白质相互作用及催化机制
J Biol Chem. 2002 Apr 19;277(16):13609-14. doi: 10.1074/jbc.M111489200. Epub 2002 Feb 6.

来自嗜热栖热菌的一种较大天然杂交蛋白的过氧化物还原酶结构域的过量生产、纯化、结晶及初步X射线分析。

Overproduction, purification, crystallization and preliminary X-ray analysis of the peroxiredoxin domain of a larger natural hybrid protein from Thermotoga maritima.

作者信息

Barbey Carole, Rouhier Nicolas, Haouz Ahmed, Navaza Alda, Jacquot Jean-Pierre

机构信息

Laboratoire de Biophysique Moléculaire, Cellulaire et Tissulaire, UMR 7033, Université Paris 13, UFR SMBH, 74 Rue Marcel Cachin, 93017 Bobigny Cedex, France.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):29-31. doi: 10.1107/S1744309107064391. Epub 2007 Dec 20.

DOI:10.1107/S1744309107064391
PMID:18097097
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2374002/
Abstract

Thermotoga maritima contains a natural hybrid protein constituted of two moieties: a peroxiredoxin domain at the N-terminus and a nitroreductase domain at the C-terminus. The peroxiredoxin (Prx) domain has been overproduced and purified from Escherichia coli cells. The recombinant Prx domain, which is homologous to bacterial Prx BCP and plant Prx Q, folds properly into a stable protein that possesses biological activity. The recombinant protein was crystallized and synchrotron data were collected to 2.9 A resolution. The crystals belonged to the tetragonal space group I422, with unit-cell parameters a = b = 176.67, c = 141.20 A.

摘要

嗜热栖热菌含有一种由两个部分组成的天然杂合蛋白

N端的过氧化物还原酶结构域和C端的硝基还原酶结构域。过氧化物还原酶（Prx）结构域已在大肠杆菌细胞中过量表达并纯化。与细菌Prx BCP和植物Prx Q同源的重组Prx结构域正确折叠成具有生物活性的稳定蛋白。该重组蛋白被结晶，并收集了分辨率为2.9 Å的同步辐射数据。晶体属于四方晶系空间群I422，晶胞参数a = b = 176.67，c = 141.20 Å。