Rothermel Markus, Schöbel Nicole, Damann Nils, Klupp Barbara G, Mettenleiter Thomas C, Hatt Hanns, Wetzel Christian H
Lehrstuhl für Zellphysiologie, Ruhr-Universität, Bochum, Germany.
J Neurovirol. 2007 Dec;13(6):579-85. doi: 10.1080/13550280701586419.
The Pseudorabies virus (PrV) strain Bartha is widely used as a tool for retrograde transneuronal tracing in mammals. Traced neurons can be identified in cell culture allowing the analysis of their physiological features ("live-cell"-tracing). Compared to PrV-Bartha, PrV-Kaplan is known for higher virulence and transsynaptic spread in both retrograde and anterograde direction. Herein we assess the authors assess PrV-Kaplan for transsynaptic anterograde "live-cell"-tracing. Following intranasal application in mice, labelled trigeminal and brainstem neurons could be identified in vitro. Detailed electrophysiological analysis indicated that viral infection did not affect neuronal properties, making PrV-Kaplan eligible for functional analysis of identified neurons within somatosensory systems.
伪狂犬病病毒（PrV）巴塔株被广泛用作哺乳动物逆行跨神经元追踪的工具。在细胞培养中可以识别被追踪的神经元，从而能够分析它们的生理特征（“活细胞”追踪）。与PrV-巴塔株相比，PrV-卡普兰株以更高的毒力以及在逆行和顺行方向上的跨突触传播而闻名。在此，我们评估PrV-卡普兰株用于跨突触顺行“活细胞”追踪的情况。在小鼠经鼻应用后，可在体外识别出标记的三叉神经和脑干神经元。详细的电生理分析表明，病毒感染不会影响神经元特性，这使得PrV-卡普兰株适用于体感系统中已识别神经元的功能分析。
