兔滑膜细胞的超微结构分析。II. 外植体和单层培养物的超微结构及形成玫瑰花结的细胞

Fine structural analysis of rabbit synovial cells. II. Fine structure and rosette-forming cells of explant and monolayer cultures.

作者信息

Krey P R, Scheinberg M A, Cohen A S

出版信息

Arthritis Rheum. 1976 May-Jun;19(3):581-92. doi: 10.1002/art.1780190310.

DOI:10.1002/art.1780190310

Abstract

The fine structure and plasma membrane receptors of explant and monolayer cells of normal rabbit synovium were studied. In explants about 10% of the cells were round and formed rosettes with IgC and C3 markers, whereas the remaining cells were stellate, resembled young fibroblasts, and had no receptors for IgG and complement. Monolayer cells looked like fibroblasts, produced fine extracellular fibrils and hyaluronate, and formed no rosettes. Thus early cultures contain both macrophages and fibroblasts but only the latter persist in monolayer.

摘要

对正常兔滑膜外植体和单层细胞的精细结构及质膜受体进行了研究。在外植体中，约10%的细胞呈圆形，与IgC和C3标记形成玫瑰花结，而其余细胞呈星状，类似年轻的成纤维细胞，且没有IgG和补体的受体。单层细胞看起来像成纤维细胞，产生精细的细胞外纤维和透明质酸盐，不形成玫瑰花结。因此，早期培养物中既有巨噬细胞也有成纤维细胞，但只有后者能在单层中持续存在。