Adamson E D
J Embryol Exp Morphol. 1976 Apr;35(2):355-67.
Extracts of embryonic mouse tissues (skeletal, cardiac and smooth muscle, and brain) were analysed by Cellogel electrophoresis for their isoenzymic distributions of three enzymes, creatine phosphokinase, aldolase and phosphoglycerate mutase. Embryonic tissues from the 12th day to the end of gestation were examined for isoenzyme transitions, and it was found that the adult forms of these enzymes appeared during gestation. Extracts from cloned teratocarcinoma cells were similarly examined in order to determine their degree of bio-chemical differentiation. Undifferentiated embryonal carcinoma cells contained only the early embryonic forms of all three enzymes, while differentiated cells formed in vivo, and in some cases in vitro, started to express the adult types of creatine phosphokinase and aldolase. Thus, biochemical parallels have been demonstrated between developing embryonic tissues and teratocarcinoma cells differentiating in vitro.
利用细胞凝胶电泳分析了胚胎期小鼠组织（骨骼肌、心肌、平滑肌和脑）中三种酶，即肌酸磷酸激酶、醛缩酶和磷酸甘油酸变位酶的同工酶分布。对妊娠第12天至妊娠末期的胚胎组织进行了同工酶转变检测，发现这些酶的成人形式在妊娠期出现。对克隆的畸胎瘤细胞提取物进行了类似检测，以确定其生化分化程度。未分化的胚胎癌细胞只含有这三种酶的早期胚胎形式，而在体内形成的分化细胞，以及在某些情况下在体外形成的分化细胞，开始表达成人类型的肌酸磷酸激酶和醛缩酶。因此，已证明发育中的胚胎组织与体外分化的畸胎瘤细胞之间存在生化相似性。
