Ling B, Liang S-F, Xu Y-H, Zhao X-Y, Tang M-H, Liu X-Y, Zhao X, Huang C-H, Chen L-J, Wei Y-Q
West China Second Hospital, and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Amino Acids. 2008 Jun;35(1):115-22. doi: 10.1007/s00726-007-0615-z. Epub 2007 Dec 21.
Honokiol (HNK) is an active component purified from Magnolia officinalis. HNK exhibits antitumor effects by inducing apoptosis and inhibiting the growth of many cancer cell lines, while proteins involved in antitumor effects in proteomic level are still unclear. In our study, HNK could inhibit HeLa cell proliferation and induce apoptosis in a concentration- and time-dependent manner. We utilized a quantitative proteomic technique termed SILAC (Stable isotope labeling with amino acids in cell culture)-MS (mass spectrometry) to study the differential proteomic profiling of HeLa cells treated by HNK. A total of 85 proteins were changed after HeLa cells were treated with 12 microg/ml HNK for 8 h, and 8 proteins showed up-regulation while 77 proteins down-regulated. The changed proteins were classified into 9 different categories, which covered a broad variety of cellular functions. In conclusion, HNK performs cytotoxicity to HeLa cells through co-operating of many proteins and different pathways.
厚朴酚（HNK）是从厚朴中纯化得到的一种活性成分。HNK通过诱导凋亡和抑制多种癌细胞系的生长发挥抗肿瘤作用，而蛋白质组水平上参与抗肿瘤作用的蛋白质仍不清楚。在我们的研究中，HNK能以浓度和时间依赖性方式抑制HeLa细胞增殖并诱导凋亡。我们利用一种称为细胞培养中氨基酸稳定同位素标记（SILAC）-质谱（MS）的定量蛋白质组学技术，研究经HNK处理的HeLa细胞的差异蛋白质组图谱。用12微克/毫升HNK处理HeLa细胞8小时后，共有85种蛋白质发生变化，其中8种蛋白质上调，77种蛋白质下调。这些变化的蛋白质被分为9个不同类别，涵盖了广泛的细胞功能。总之，HNK通过多种蛋白质和不同途径的协同作用对HeLa细胞发挥细胞毒性作用。
