水牛（Bubalus bubalis）1号染色体（BBU1）的辐射杂种图谱。
A radiation hybrid map of river buffalo (Bubalus bubalis) chromosome 1 (BBU1).
作者信息
Miziara M N, Goldammer T, Stafuzza N B, Ianella P, Agarwala R, Schaffer A A, Elliott J S, Riggs P K, Womack J E, Amaral M E J
机构信息
Departamento Biologia, UNESP - São Paulo State University, IBILCE, São Jose Rio Preto, Brazil.
出版信息
Cytogenet Genome Res. 2007;119(1-2):100-4. doi: 10.1159/000109625. Epub 2007 Dec 14.
The largest chromosome in the river buffalo karyotype, BBU1, is a submetacentric chromosome with reported homology between BBU1q and bovine chromosome 1 and between BBU1p and BTA27. We present the first radiation hybrid map of this chromosome containing 69 cattle derived markers including 48 coding genes, 17 microsatellites and four ESTs distributed in two linkage groups spanning a total length of 1330.1 cR(5000). The RH map was constructed based on analysis of a recently developed river buffalo-hamster whole genome radiation hybrid (BBURH(5000)) panel. The retention frequency of individual markers across the panel ranged from 17.8 to 52.2%. With few exceptions, the order of markers within linkage groups is identical to the order established for corresponding cattle RH maps. The BBU1 map provides a starting point for comparison of gene order rearrangements between river buffalo chromosome 1 and its bovine homologs.
河流型水牛核型中最大的染色体BBU1是一条亚中着丝粒染色体，据报道BBU1q与牛的1号染色体以及BBU1p与牛的27号染色体之间存在同源性。我们展示了该染色体的首张辐射杂种图谱，其中包含69个牛源标记，包括48个编码基因、17个微卫星和4个EST，分布在两个连锁群中，总长为1330.1 cR(5000)。该辐射杂种图谱是基于对最近构建的河流型水牛-仓鼠全基因组辐射杂种(BBURH(5000))板的分析构建的。各个标记在该板中的保留频率在17.8%至52.2%之间。除少数例外，连锁群内标记的顺序与相应牛辐射杂种图谱确定的顺序相同。BBU1图谱为比较河流型水牛1号染色体与其牛同源染色体之间的基因顺序重排提供了一个起点。
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