膜联蛋白p68的微晶：从副晶到晶体的转变以及通过电子显微镜和图像重建确定的分子堆积
Microcrystals of the annexin, p68: paracrystal to crystal transition and molecular packing as determined by electron microscopy and image reconstruction.
作者信息
Gabriel B L, Taylor K, Creutz C E, Kretsinger R H
机构信息
Department of Pharmacology, University of Virginia, Charlottesville 22901.
出版信息
J Struct Biol. 1991 Aug;107(1):29-37. doi: 10.1016/1047-8477(91)90028-u.
The calcium-sensitive, membrane-binding annexin, p68, has been crystallized from solutions of polyethylene glycol and ammonium sulfate. Our electron microscopy and X-ray diffraction data indicate that p68 crystals are tetragonal, in space group P4(1), and have unit cell dimensions of a = b = 68.4 A and c = 209.6 A. The mechanism of crystallization from polyethylene glycol involves a transition from a paracrystalline form to ordered crystals by lateral reordering of chains of molecules extended along the c axis. These chains are directional and might reflect a mechanism whereby the two different ends of (chains of) the p68 molecules interact with different membranes.
对钙敏感的膜结合膜联蛋白p68已从聚乙二醇和硫酸铵溶液中结晶出来。我们的电子显微镜和X射线衍射数据表明，p68晶体为四方晶系，空间群为P4(1)，晶胞参数为a = b = 68.4 Å，c = 209.6 Å。从聚乙二醇中结晶的机制涉及从准晶形式通过沿c轴延伸的分子链的侧向重排转变为有序晶体。这些链是有方向性的，可能反映了一种机制，即p68分子（链）的两个不同末端与不同的膜相互作用。
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