Bartolucci C, Cellai L, Corradini C, Corradini D, Lamba D, Veloná I
Institute of Structural Chemistry, Giordano Giacomello, CNR, Rome, Italy.
Int J Tissue React. 1991;13(6):311-7.
Lysosomal hyaluronidase is responsible for the degradation of hyaluronan, a component of the extracellular matrix, in degenerative disorders of the joints. It has been hypothesized that the administration of chondroitin sulfate (both a component of the extracellular matrix and a substrate for hyaluronidase) could compete for this enzyme and reduce the degradation process. The present study shows that a mixture of chondroitin 4-sulfate and chondroitin 6-sulfate is a good competitor of hyaluronan for hyaluronidase. The digestion of hyaluronan is reduced in proportion to the amount of competing chondroitin. The competitive ability is dependent on the 4-sulfate, 6-sulfate composition of the chondroitin mixture. Mixtures richer in the 4-sulfate isomer are more effective. The enzymatic reactions have been monitored by HPLC and PAGE.
溶酶体透明质酸酶负责在关节退行性疾病中降解细胞外基质的成分透明质酸。据推测，给予硫酸软骨素（既是细胞外基质的成分又是透明质酸酶的底物）可以与这种酶竞争并减少降解过程。本研究表明，硫酸软骨素4-硫酸盐和硫酸软骨素6-硫酸盐的混合物是透明质酸与透明质酸酶竞争的良好竞争者。透明质酸的消化与竞争性硫酸软骨素的量成比例减少。竞争能力取决于硫酸软骨素混合物的4-硫酸盐、6-硫酸盐组成。富含4-硫酸盐异构体的混合物更有效。酶促反应已通过高效液相色谱法和聚丙烯酰胺凝胶电泳进行监测。
