从木豆种子中纯化脲酶及其在生物传感器构建中的应用。

Urease purification from the seeds of Cajanus cajan and its application in a biosensor construction.

作者信息

Pandey P C, Pandey V

机构信息

Department of Chemistry, Banaras Hindu University, Varanasi, India.

出版信息

Appl Biochem Biotechnol. 1991 Dec;31(3):247-51. doi: 10.1007/BF02921751.

DOI:10.1007/BF02921751

PMID:1818537

Abstract

Urease has been purified from the seeds of Cajanus Cajan. The purification process involves three solvent extraction steps followed by DEAE-cellulose column chromatography. The specific activity of the purified enzyme is found to be 1920 U/mg with the recovery of 8%. The application of the purified enzyme in a biosensor construction is discussed.

摘要

脲酶已从木豆种子中纯化出来。纯化过程包括三个溶剂萃取步骤，随后进行DEAE-纤维素柱色谱法。纯化后的酶的比活性为1920 U/mg，回收率为8%。文中讨论了纯化后的酶在生物传感器构建中的应用。