Stutte Susanne, Jux Bettina, Esser Charlotte, Förster Irmgard
Division of Molecular Immunology, Institut für Umweltmedizinische Forschung, Heinrich Heine University, Düsseldorf, Germany.
J Invest Dermatol. 2008 Jun;128(6):1470-5. doi: 10.1038/sj.jid.5701228. Epub 2008 Jan 10.
Langerhans cells (LCs) and dermal dendritic cells (dDCs) are the professional antigen-presenting cells of the skin. Recently, their immunogenic versus tolerogenic role has come under re-investigation. LCs are distinguished from dDCs by Langerin (CD207) staining or by detection of Birbeck granules. However, for in vitro experiments it is desirable to have a simple and robust flow cytometric demarcation of both cell types. We show here that CD24a is expressed on LCs but not on dDCs isolated directly from the skin. Moreover, in combination with major histocompatibility complex class II (MHCII), CD24a expression levels distinguish LCs from dDCs in skin-draining lymph nodes after antigen activation and migration. High expression of CD24a correlated strictly with CD207 expression. MHCII(high) cells were unique for skin-draining lymph nodes and were shown to be the only cells carrying antigen after FITC painting of the skin. CD24a expression levels further differentiated LCs and dDCs in the MHCII(high) population. As staining for CD24a does not require fixation of cells, CD24a-stained cells can be used for in vitro experiments to analyze and compare the functional roles and properties of dDCs and LCs.
朗格汉斯细胞（LCs）和真皮树突状细胞（dDCs）是皮肤中的专职抗原呈递细胞。最近，它们在免疫原性与耐受性方面的作用再次受到研究。通过朗格素（CD207）染色或检测伯贝克颗粒可将LCs与dDCs区分开来。然而，对于体外实验而言，希望有一种简单且可靠的流式细胞术方法来区分这两种细胞类型。我们在此表明，CD24a在LCs上表达，而在直接从皮肤分离的dDCs上不表达。此外，与主要组织相容性复合体II类（MHCII）结合后，CD24a的表达水平在抗原激活和迁移后可区分皮肤引流淋巴结中的LCs和dDCs。CD24a的高表达与CD207的表达严格相关。MHCII（高）细胞在皮肤引流淋巴结中是独特的，并且在皮肤用异硫氰酸荧光素（FITC）染色后显示是唯一携带抗原的细胞。CD24a的表达水平在MHCII（高）群体中进一步区分了LCs和dDCs。由于对CD24a的染色不需要固定细胞，因此用CD24a染色的细胞可用于体外实验，以分析和比较dDCs和LCs的功能作用及特性。
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