Mejía Carmen, García-Alcocer Guadalupe, Berumen Laura C, Rosas-Arellano Abraham, Miledi Ricardo, Martínez-Torres Ataúlfo
Instituto de Neurobiología, Departamento de Neurobiología Molecular y Celular, Universidad Nacional Autónoma de México-Campus Juriquilla, (Boulevar) Universitario 3001, Querétaro, 76230, Mexico.
Neurosci Lett. 2008 Feb 13;432(1):1-6. doi: 10.1016/j.neulet.2007.11.062. Epub 2007 Dec 14.
In the present study, we provide evidence for the expression of all three GABA(C) receptor rho subunits through development of the rat cerebellum. Injection of cerebellum mRNA into frog oocytes gave rise to the expression of both GABA(A) and GABA(C) receptors. qRT-PCR of RNA isolated from postnatal developing cerebella showed that the expression of each rho subunit is relatively low, with a relative comparative expression of rho3>rho1>rho2. In situ hybridization and immunohistochemistry revealed a limited distribution of GABA(C) receptors in the Purkinje and Golgi neurons whereas electron microscopy detected the rho1 and rho2 subunits in the soma and dendritic tree of the Purkinje cells. The expression of GABA(C) receptors in the cerebellum adds a new dimension to the regulation of GABAergic neurotransmission and suggests further experiments to determine their functional consequences.
在本研究中，我们通过大鼠小脑发育过程的研究，为所有三种GABA(C)受体rho亚基的表达提供了证据。将小脑mRNA注射到蛙卵母细胞中可导致GABA(A)和GABA(C)受体的表达。对出生后发育中小脑分离的RNA进行qRT-PCR分析表明，每个rho亚基的表达相对较低，rho3>rho1>rho2呈相对比较表达。原位杂交和免疫组织化学显示GABA(C)受体在浦肯野神经元和高尔基神经元中分布有限，而电子显微镜在浦肯野细胞的胞体和树突中检测到rho1和rho2亚基。小脑GABA(C)受体的表达为GABA能神经传递的调节增添了新的层面，并提示进一步实验以确定其功能后果。
