胃肠道黏膜内肿瘤中血管生成和淋巴管生成的分布情况。

Distributions of angiogenesis and lymphangiogenesis in gastrointestinal intramucosal tumors.

作者信息

Gao Yan, Zhong Wei-Xia, Mu Dian-Bin, Yuan Yin-Ping, Zhang Yu-Hua, Yu Jin-Ming, Sun Lan-Ping, Wang Lei, Li Yu-Hui, Zhang Jian-Bo, Zhao Yan, Cai Shu-Ping, Zhou Geng-Yin

机构信息

Department of Pathology, Shandong Cancer Hospital, Shandong Academy of Medical Science, 440 Jiyan Road, Jinan, Shandong, PR China.

出版信息

Ann Surg Oncol. 2008 Apr;15(4):1117-23. doi: 10.1245/s10434-007-9752-6. Epub 2008 Jan 18.

DOI:10.1245/s10434-007-9752-6

PMID:18202892

Abstract

BACKGROUND

Although angiogenesis and lymphangiogenesis in gastrointestinal cancers has been investigated in many studies, their distribution characteristics in gastrointestinal intramucosal tumors have not been well addressed.

METHODS

We evaluated the blood microvessel density (BMVD) and lymphatic microvessel density (LMVD) by immunostaining with monoclonal antibodies of CD34 and D2-40 in 37 patients with stomach intramucosal carcinoma and 28 patients with colorectal intramucosal neoplasia. Microvessels with endothelial cells labeled by CD34 but not by D2-40 were recognized as blood microvessels; and microvessels with endothelial cells labeled by both CD34 and D2-40 were recognized as lymphatic vessels. Furthermore, the relationships between expression of vascular endothelial growth factor (VEGF), VEGF-C, and BMVD, LMVD were investigated as well.

RESULTS

The LMVD was significantly higher in peritumoral tissues than in corresponding normal tissues in gastrointestinal intramucosal tumors (20.87 versus 14.56, P = 0.003). However, there was no significant difference in the BMVD between peritumoral tissues and corresponding normal tissues (P = 0.166). The BMVD in peritumoral tissues was higher in patients with lymph node metastases than in patients without lymph nodes metastases (P = 0.047). Our results did not show significant association between VEGF, VEGF-C and BMVD, LMVD.

CONCLUSIONS

Our results suggested that the increase of lymphangiogenesis seems superior to the increase of angiogenesis in gastrointestinal intramucosal tumors.

摘要

背景

尽管许多研究已对胃肠道癌中的血管生成和淋巴管生成进行了调查，但它们在胃肠道黏膜内肿瘤中的分布特征尚未得到充分研究。

方法

我们采用CD34和D2-40单克隆抗体免疫染色法，对37例胃黏膜内癌患者和28例结直肠黏膜内瘤变患者的血液微血管密度（BMVD）和淋巴管微血管密度（LMVD）进行了评估。内皮细胞被CD34标记但未被D2-40标记的微血管被识别为血液微血管；内皮细胞同时被CD34和D2-40标记的微血管被识别为淋巴管。此外，还研究了血管内皮生长因子（VEGF）、VEGF-C的表达与BMVD、LMVD之间的关系。

结果

胃肠道黏膜内肿瘤的瘤周组织中LMVD显著高于相应正常组织（分别为20.87和14.56，P = 0.003）。然而，瘤周组织与相应正常组织之间的BMVD无显著差异（P = 0.166）。有淋巴结转移患者的瘤周组织中BMVD高于无淋巴结转移患者（P = 0.047）。我们的结果未显示VEGF、VEGF-C与BMVD、LMVD之间存在显著关联。