Webb Diane, Gagnon Marthe Monique, Rose Tom
Department of Environmental Biology, Curtin University of Technology, Bentley, WA, Australia.
Environ Toxicol. 2008 Feb;23(1):68-76. doi: 10.1002/tox.20307.
The yellowtail trumpeter (Amniataba caudavittata) is an estuarine-dependent omnivorous fish found in the Swan-Canning Estuary, Western Australia. Thirty five fish were injected with either the polycyclic aromatic hydrocarbon, benzo[a]pyrene (B[a]P), the synthetic flavenoid beta-naphthoflavone (BNF), or used as controls. The fish were then sampled at 3 and 7 days postinjection. Induction of the enzyme ethoxyresorufin O-deethylase (EROD) activity was nonsignificant while ethoxycoumarin O-deethylase (ECOD) activity induction differed amongst treatments. A high interindividual variability in the EROD activity was observed. The measurement of sorbitol dehydrogenase in the serum (s-SDH) was elevated (BNF 2.2 times and B[a]P 3.2 times the control fish) demonstrating that liver cell damage had occurred. Increases in biliary metabolites of both B[a]P-type and pyrene-type (19 times and 3.4 times the controls respectively) indicated that detoxification of pyrene-type compounds had taken place. Fish of the Terapontidae family, such as the yellowtail trumpeter, were found to be suitable for biomonitoring the health of the Swan-Canning Estuary. A combination of ECOD activity, s-SDH, and the measurement of biliary metabolites represents a suitable suite of biomarkers for environmental monitoring of the sublethal effects of PAH pollution in these fish.
黄斑笛鲷（Amniataba caudavittata）是一种依赖河口生存的杂食性鱼类，在西澳大利亚的天鹅 - 坎宁河口被发现。35条鱼分别被注射了多环芳烃苯并[a]芘（B[a]P）、合成类黄酮β-萘黄酮（BNF），或用作对照。然后在注射后3天和7天对鱼进行采样。乙氧基异吩恶唑酮 - O - 脱乙基酶（EROD）活性的诱导不显著，而乙氧基香豆素 - O - 脱乙基酶（ECOD）活性的诱导在不同处理之间存在差异。观察到EROD活性存在较高的个体间变异性。血清山梨醇脱氢酶（s - SDH）的测量值升高（BNF组是对照鱼的2.2倍，B[a]P组是对照鱼的3.2倍），表明发生了肝细胞损伤。B[a]P型和芘型胆汁代谢物的增加（分别是对照组的19倍和3.4倍）表明芘型化合物发生了解毒作用。发现笛鲷科鱼类，如黄斑笛鲷，适合用于生物监测天鹅 - 坎宁河口的健康状况。ECOD活性、s - SDH以及胆汁代谢物的测量相结合，代表了一套适用于环境监测这些鱼类中PAH污染亚致死效应的生物标志物。
