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低通气水平和高通气水平条件下H2O2对耐氧氧化微小杆菌T-2-2T生长及过氧化氢酶活性的影响

Effects of H2O2 under low- and high-aeration-level conditions on growth and catalase activity in Exiguobacterium oxidotolerans T-2-2T.

作者信息

Takebe Fumihiko, Hara Isao, Matsuyama Hidetoshi, Yumoto Isao

机构信息

Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology, 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.

出版信息

J Biosci Bioeng. 2007 Dec;104(6):464-9. doi: 10.1263/jbb.104.464.

Abstract

The effects of H2O2 under low- and high-aeration-level conditions on growth and catalase activity in Exiguobacterium oxidotolerans T-2-2T were investigated. Continuous addition of 5-200 mM H2O2 to the culture medium from the mid-exponential growth phase enhanced the growth of the strain under the low-aeration-level condition, whereas the addition of 5-50 mM H2O2 decreased intracellular specific catalase activity and extracellular total catalases activity. The detection of extracellular catalase by the cells and the decrease in intracellular specific catalase activity and extracellular total catalase activity under the high-aeration-level condition account for the stimulation of growth by the introduced H2O2 and the decrease in catalase activities induced by O(2) from H2O2 in the medium. On the other hand, the addition of H2O2 to the medium prior to the initiation of growth inhibited the growth but increased the specific activity of intracellular catalase in the stationary growth phase. Strain T-2-2T grew when 10 mM H2O2 was added to the medium prior to growth. However, the growth was completely inhibited by the catalase inhibitor 3-amino-1,2,4-triazole (3-AT). The continuous addition of H2O2 at an appropriate concentration from prior to the initiation of growth to the stationary growth phase under the low-aeration-level condition resulted in higher intracellular specific catalase activity and cell growth rate than single H2O2 addition prior to growth.

摘要

研究了低通气水平和高通气水平条件下过氧化氢对耐氧化微小杆菌T-2-2T生长及过氧化氢酶活性的影响。从中指数生长期开始向培养基中持续添加5-200 mM过氧化氢可促进该菌株在低通气水平条件下的生长,而添加5-50 mM过氧化氢会降低细胞内过氧化氢酶比活性和细胞外总过氧化氢酶活性。在高通气水平条件下,细胞对细胞外过氧化氢酶的检测以及细胞内过氧化氢酶比活性和细胞外总过氧化氢酶活性的降低,解释了引入的过氧化氢对生长的刺激作用以及培养基中过氧化氢分解产生的氧气对过氧化氢酶活性的诱导降低。另一方面,在生长开始前向培养基中添加过氧化氢会抑制生长,但会增加稳定生长期细胞内过氧化氢酶的比活性。在生长前向培养基中添加10 mM过氧化氢时,菌株T-2-2T能够生长。然而,过氧化氢酶抑制剂3-氨基-1,2,4-三唑(3-AT)完全抑制了生长。在低通气水平条件下,从生长开始前到稳定生长期持续添加适当浓度的过氧化氢,与生长前单次添加过氧化氢相比,可导致更高的细胞内过氧化氢酶比活性和细胞生长速率。

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