铁、水杨酸盐和锌对体内金属硫蛋白及脂质过氧化的影响。

Effects of Fe, salicylate and Zn on metallothionein and lipid peroxidation in vivo.

作者信息

Günther T, Grossrau R, Höllriegl V, Vormann J

机构信息

Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Fed. Rep. of Germany.

出版信息

J Trace Elem Electrolytes Health Dis. 1991 Jun;5(2):95-100.

PMID:1821716

Abstract

Control rats and rats pretreated with two i.p. injections of 15 mg/kg Fe, given as FeCl3, received either one oral dose of 700 mg/kg salicylic acid, given as Na salicylate, or 100 mumol ZnCl2/kg s.c., or salicylate and ZnCl2. In the rats given Fe this treatment was simultaneous with the second iron injection. Salicylate and Fe alone caused a small increase in lipid peroxidation (LPO) measured as malondialdehyde formation in the liver by means of the thiobarbiturate method. When given together, however, Fe and salicylate caused a drastic increase of LPO in liver, which was reduced by the simultaneous injection of ZnCl2. Hepatotoxicity as determined histochemically by lipid storage paralleled LPO. The protective effect of Zn on LPO and hepatotoxicity was not correlated to metallothionein induction in liver. The protective effect of Zn can be explained by the competition of Fe with Zn for binding to salicylate which reduces Fe-salicylate-induced LPO and hepatotoxicity.

摘要

对照组大鼠以及经腹腔注射两次15mg/kg氯化铁（以FeCl₃形式给药）预处理的大鼠，分别接受一次口服剂量为700mg/kg的水杨酸钠、皮下注射100μmol/kg氯化锌，或同时接受水杨酸钠和氯化锌。对于接受铁处理的大鼠，该处理与第二次铁注射同时进行。单独使用水杨酸盐和铁会使脂质过氧化（LPO）略有增加，通过硫代巴比妥酸法测定肝脏中丙二醛的形成来衡量脂质过氧化情况。然而，当铁和水杨酸盐同时使用时，会导致肝脏中LPO急剧增加，而同时注射氯化锌可使其降低。通过脂质储存组织化学测定的肝毒性与LPO情况相似。锌对LPO和肝毒性的保护作用与肝脏中金属硫蛋白的诱导无关。锌的保护作用可以通过铁与锌竞争结合水杨酸盐来解释，这减少了铁 - 水杨酸盐诱导的LPO和肝毒性。