Suskind Anne, Hayner-Buchan Alida, Feustel Paul J, Kogan Barry A
Urological Institute of Northeastern New York, Division of Urology, Albany Medical College, Albany, NY 12208, USA.
BJU Int. 2008 Jun;101(11):1441-5. doi: 10.1111/j.1464-410X.2007.07406.x. Epub 2008 Jan 24.
To evaluate whether semiquantitatively assessing the histological degree of fibrosis provides equivalent information to a detailed histological analysis of undescended testes in children.
We reviewed retrospectively the histological and clinical data from 86 consecutive orchidopexies. Biopsies fixed in Bouin's solution were evaluated histologically for tubular diameter, germ cell number and fibrosis. Fibrosis was assessed semiquantitatively by a pathologist reviewing sections stained with Masson's trichrome. The median (range) age of the patients was 1.9 (0.5-13.5) years, with 29 aged <1 year and 57 aged >1 year.
There were 65 palpable and 21 impalpable testes; 62 were inguinal and 13 were intra-abdominal. Testicular biopsies showed smaller tubules and fewer germ cells in patients aged >1 year, but there were no significant differences based on the location of the testis on physical examination or at the time of operation. The amount of fibrosis correlated well with tubular diameter and germ cell counts.
Fibrosis can be assessed easily and correlates well with more complex forms of histological evaluation of testicular health in children.
评估对纤维化的组织学程度进行半定量评估是否能提供与对儿童隐睾进行详细组织学分析同等的信息。
我们回顾性分析了连续86例睾丸固定术的组织学和临床数据。对用Bouin液固定的活检组织进行组织学评估，观察其管径、生殖细胞数量和纤维化情况。由一名病理学家对用Masson三色染色的切片进行半定量评估纤维化程度。患者的年龄中位数（范围）为1.9（0.5 - 13.5）岁，其中29例年龄小于1岁，57例年龄大于1岁。
可触及的睾丸有65个，不可触及的有21个；腹股沟型62个，腹腔型13个。睾丸活检显示，年龄大于1岁的患者，其小管较小，生殖细胞较少，但根据体格检查或手术时睾丸的位置并无显著差异。纤维化程度与管径和生殖细胞计数密切相关。
纤维化易于评估，且与对儿童睾丸健康进行的更复杂的组织学评估密切相关。
