通过连接核酸碱基配对相互作用实现受体介导的小干扰RNA递送。
Receptor-mediated delivery of siRNAs by tethered nucleic acid base-paired interactions.
作者信息
Zhang Kexiong, Wang Qiaoqiao, Xie Yanhua, Mor Gil, Sega Emanuela, Low Philip S, Huang Yingqun
机构信息
Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06511, USA.
出版信息
RNA. 2008 Mar;14(3):577-83. doi: 10.1261/rna.739308. Epub 2008 Jan 24.
Abstract
We report a new strategy for cell-type-specific delivery of functional siRNAs into cells. The method involves the noncovalent attachment of siRNAs to ligand-conjugated oligodeoxynucleotides via nucleic acid base-paired interactions. The resulting complexes can be directly applied to cells, leading to specific cellular uptake and gene silencing. The method is simple, economical, and can be easily adapted for other cell surface receptors. Here we show the application of this method for the delivery of siRNAs to folate receptor-expressing cells.
摘要
我们报告了一种将功能性小干扰RNA（siRNA）细胞类型特异性递送至细胞内的新策略。该方法涉及通过核酸碱基配对相互作用将siRNA非共价连接至配体偶联的寡脱氧核苷酸。所得复合物可直接应用于细胞，从而实现特异性细胞摄取和基因沉默。该方法简单、经济，并且能够轻松适用于其他细胞表面受体。在此，我们展示了该方法在将siRNA递送至表达叶酸受体的细胞中的应用。
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