Wang Chun-Chin, Fang Guor-Cheng, Lee LienYao
Waste and Biology Treatment Laboratory, Department of Environmental Engineering, HungKuang University, Sha-Lu, Taichung 433, Taiwan.
Toxicol Ind Health. 2007 Apr;23(3):133-9. doi: 10.1177/0748233707078741.
Suspended particles, of which bioaerosols are one type, constitute one of the main reasons to cause severe air quality in Taiwan. Bioaerosols include allergens such as fungi, bacteria, actinomycetes, arthropods and protozoa, as well as microbial products such as mycotoxins, endotoxins and glucans. When allergens and microbial products are suspended in the air, local air quality will be influenced severely. In addition, when the particle size is small enough to pass through the respiratory tract entering the human body, the health of the local population is also threatened. Therefore, the purpose of this study attempted to understand the concentration and types of bacteria during summer period at four sampling sites in Taichung city, central Taiwan. The results indicated that total average bacterial concentration by using R2A medium incubated for 48 h were 7.3 x 10(2) and 1.2 x 10(3) cfu/m3 for Chung-Ming elementary sampling site during daytime and night-time period of summer season. In addition, total average bacterial concentration by using R2A medium incubated for 48 h were 2.2 x 10(3) and 2.5 x 10(3) cfu/m3 for Taichung refuse incineration plant sampling site during daytime and night-time period of summer season. As for Rice Field sampling site during daytime and night-time period of summer season, the results also reflected that the total average bacterial concentration by using R2A medium incubated for 48 h were 3.4 x 10(3) and 3.5 x 10(3) cfu/m3. Finally, total average bacterial concentration by using R2A medium incubated for 48 h were 1.6 x 10(3) and 1.9 x 10(3) cfu/m3 for Central Taiwan Science Park sampling site during daytime and night-time period of summer season. Moreover, the average bacterial concentration increased as the incubated time in a growth medium increased for particle sizes of 0.65-1.1, 1.1-2.1, 2.1-3.3, 3.3-4.7 and 4.7-7.0 microm. The total average bacterial concentration has no significant difference for day and night sampling period at any sampling site for the expression of bacterial concentration in term of order. The high average bacterial concentration was found in the particle size of 0.53-0.71 mm (average bioaerosol size was in the range of 2.1-4.7 microm) for each sampling site. Besides, there were exceeded 20 kinds of bacteria for each sampling site and the bacterial shape were rod, coccus and filamentous.
悬浮颗粒(生物气溶胶是其中一种类型)是导致台湾空气质量严重恶化的主要原因之一。生物气溶胶包括过敏原,如真菌、细菌、放线菌、节肢动物和原生动物,以及微生物产物,如霉菌毒素、内毒素和葡聚糖。当过敏原和微生物产物悬浮在空气中时,当地空气质量会受到严重影响。此外,当颗粒小到足以穿过呼吸道进入人体时,当地居民的健康也会受到威胁。因此,本研究旨在了解台湾中部台中市四个采样点夏季期间细菌的浓度和种类。结果表明,夏季白天和夜间,在忠明小学采样点,使用R2A培养基培养48小时后的细菌总平均浓度分别为7.3×10²和1.2×10³ cfu/m³。此外,夏季白天和夜间,在台中垃圾焚烧厂采样点,使用R2A培养基培养48小时后的细菌总平均浓度分别为2.2×10³和2.5×10³ cfu/m³。至于夏季白天和夜间的稻田采样点,结果也显示,使用R2A培养基培养48小时后的细菌总平均浓度分别为3.4×10³和3.5×10³ cfu/m³。最后,夏季白天和夜间,在台湾中部科学园区采样点,使用R2A培养基培养48小时后的细菌总平均浓度分别为1.6×10³和1.9×10³ cfu/m³。此外,对于粒径为0.65 - 1.1、1.1 - 2.1、2.1 - 3.3、3.3 - 4.7和4.7 - 7.0微米的颗粒,平均细菌浓度随着在生长培养基中的培养时间增加而增加。在任何采样点,以细菌浓度的量级表示,白天和夜间采样期间的细菌总平均浓度没有显著差异。在每个采样点,粒径为0.53 - 0.71毫米(平均生物气溶胶粒径在2.1 - 4.7微米范围内)时发现平均细菌浓度较高。此外,每个采样点有超过20种细菌,细菌形态为杆状、球状和丝状。