使用蛋白A免疫反应器的流动注射免疫分析
Flow injection immunoassay using a protein A immunoreactor.
作者信息
Miller J N, Palmer D A, French M T
机构信息
Department of Chemistry, Loughborough University, Leicestershire, UK.
出版信息
J Pharm Biomed Anal. 1991;9(10-12):1115-20. doi: 10.1016/0731-7085(91)80052-b.
PMID:1822181
Abstract
Competitive immunoassays have been developed for the immunosuppressant cyclosporin A and the anti-asthmatic drug theophylline utilizing identical controlled pore glass-protein A microcolumns and flow injection techniques. For cyclosporin A the assay was based on a monoclonal antibody with fluorescence detection whilst for theophylline sheep antiserum and electrochemical detection was used.
摘要
利用相同的可控孔径玻璃-蛋白A微柱和流动注射技术，已开发出用于免疫抑制剂环孢素A和抗哮喘药物茶碱的竞争性免疫测定法。对于环孢素A，该测定法基于一种带有荧光检测的单克隆抗体，而对于茶碱，则使用绵羊抗血清和电化学检测。
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