用于分析细胞色素c'功能的一氧化氮递送

Delivery of nitric oxide for analysis of the function of cytochrome c'.

作者信息

Cole Lindsay J, Huston Wilhelmina M, Moir James W B

机构信息

Department of Biology, University of York, Heslington, York, United Kingdom.

出版信息

Methods Enzymol. 2008;436:21-33. doi: 10.1016/S0076-6879(08)36002-9.

DOI:10.1016/S0076-6879(08)36002-9

PMID:18237625

Abstract

On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c'), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'.

摘要

在将一氧化氮（NO）输送到蛋白质样品（如细胞色素c'）以进行光谱实验时，避免暴露于氧气并去除NO气体中的污染物非常重要。我们描述了一些用于细胞色素c'的稳态紫外/可见分光光度法和预稳态停流分光光度法分析的技术。