脂肪酶在反胶束中的催化性能研究。

A study on the catalytic performance of lipase in reverse micelles.

作者信息

Xu J, Mao Q, Ying X, Hu Y

机构信息

Thermodynamics Research Laboratory, East China University of Chemical Technology, Shanghai.

出版信息

Chin J Biotechnol. 1991;7(4):301-7.

PMID:1824243

Abstract

The hydrolysis of olive oil catalyzed by Candida rugosa lipase solubilized in "H2O/AOT/isooctane" reverse micellar solution has been studied. The optimal conditions observed for lipase in reverse micelles were t = 25 degrees C, pH = 7.9, and R = [H2O]/[AOT] = 10. Lipase in reverse micelles is found to be quite stable. For example, after being incubated at 33 degrees C for 12 hours in AOT/isooctane with R = 5.4, the residual activity of lipase remains as high as 89%. No inhibition was observed even though the substrate concentration was increased up to 50% (v/v), and it was inhibited slightly when 40 mM Cu2+, Co2+ or Mn2+ ions were in the water pools.

摘要

研究了溶解于“H₂O/AOT/异辛烷”反胶束溶液中的皱褶假丝酵母脂肪酶催化橄榄油水解的情况。反胶束中脂肪酶的最佳条件为t = 25℃，pH = 7.9，且R = [H₂O]/[AOT] = 10。发现反胶束中的脂肪酶相当稳定。例如，在R = 5.4的AOT/异辛烷中于33℃孵育12小时后，脂肪酶的残余活性仍高达89%。即使底物浓度增加至50%（v/v）也未观察到抑制作用，而当水池中有40 mM Cu²⁺、Co²⁺或Mn²⁺离子时，脂肪酶会受到轻微抑制。