Eguchi Ryoji, Ishihara Akinori, Yamauchi Kiyoshi
Graduate School of Science and Engineering; Shizuoka University, Shizuoka, 422-8529, Japan.
Comp Biochem Physiol C Toxicol Pharmacol. 2008 Apr;147(3):345-50. doi: 10.1016/j.cbpc.2008.01.002. Epub 2008 Jan 12.
The association of suspected endocrine-disrupting chemicals (EDCs), diethylstilbestrol (DES), ioxynil and pentachlorophenol (PCP), with chicken serum proteins was investigated in relation to thyroid system disruption. All of these chemicals strongly inhibited l-[(125)I]thyroxine ([(125)I]T(4)) binding to purified transthyretin (TTR) whereas PCP was less potent inhibitor than DES and ioxynil of [(125)I]T(4) binding to diluted whole chicken serum. This result suggested that PCP interacted with serum proteins other than TTR in whole chicken serum. Following the incubation of chicken serum with each chemical (final concentrations 0.25-1.0 microM), serum proteins were fractionated by gel filtration chromatography (Cellulofine GCL-1000) and affinity chromatography (human retinol-binding protein coupled to Sepharose 4B). Although all chemicals were detected in the gel filtration chromatography 50-100 kDa fractions, DES and ioxynil, but not PCP, were co-eluted with TTR during affinity chromatography. Our results indicated that a significant proportion of DES and ioxynil, but a low proportion of PCP, interacted with TTR in whole chicken serum.
研究了疑似内分泌干扰化学物质（EDC）己烯雌酚（DES）、碘苯腈和五氯苯酚（PCP）与鸡血清蛋白的关联及其对甲状腺系统的干扰作用。所有这些化学物质均强烈抑制L-[(125)I]甲状腺素（[(125)I]T4）与纯化的转甲状腺素蛋白（TTR）的结合，而在[(125)I]T4与稀释的全鸡血清结合方面，PCP的抑制效力低于DES和碘苯腈。这一结果表明，PCP与全鸡血清中TTR以外的血清蛋白相互作用。将鸡血清与每种化学物质（终浓度0.25 - 1.0 microM）孵育后，通过凝胶过滤色谱法（Cellulofine GCL - 1000）和亲和色谱法（与人视黄醇结合蛋白偶联的琼脂糖4B）对血清蛋白进行分离。尽管在凝胶过滤色谱法的50 - 100 kDa级分中均检测到所有化学物质，但在亲和色谱过程中，DES和碘苯腈与TTR共洗脱，而PCP则没有。我们的结果表明，在全鸡血清中，相当一部分DES和碘苯腈与TTR相互作用，而PCP与TTR相互作用的比例较低。
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