用于恶性黑色素瘤冰冻切片的20分钟快速MART-1免疫染色。
The 20-minute rapid MART-1 immunostain for malignant melanoma frozen sections.
作者信息
Kimyai-Asadi Arash, Ayala Gabriel B, Goldberg Leonard H, Vujevich Justin, Jih Ming H
机构信息
DermSurgery Associates, Houston, Texas 77030, USA.
出版信息
Dermatol Surg. 2008 Apr;34(4):498-500. doi: 10.1111/j.1524-4725.2007.34095.x. Epub 2008 Jan 31.
BACKGROUND
Immunohistochemical staining has been used to help detect malignant melanoma on Mohs surgery frozen sections. Previous investigators have developed protocols for reliable MART-1 immunostaining of frozen sections, but these protocols are time-consuming.
OBJECTIVE
The objective was to report a rapid 20-minute MART-1 immunostaining protocol for frozen sections.
METHODS
The protocol was utilized on 30 melanomas treated with Mohs micrographic surgery.
RESULTS
The stain clearly highlighted normal background melanocytes, as well as melanocytic hyperplasia and malignant melanoma.
CONCLUSIONS
The 20-minute protocol provides a rapid and reliable method for immunostaining of malignant melanoma. The availability of more rapid immunostaining methods improves efficiency of the Mohs laboratory and significantly reduces patient and physician waiting time. The authors have indicated no significant interest with commercial supporters.
背景
免疫组织化学染色已被用于在莫氏手术冰冻切片上帮助检测恶性黑色素瘤。先前的研究人员已开发出用于冰冻切片可靠的MART-1免疫染色方案，但这些方案耗时较长。
目的
目的是报告一种用于冰冻切片的快速20分钟MART-1免疫染色方案。
方法
该方案应用于30例接受莫氏显微外科手术治疗的黑色素瘤。
结果
该染色清晰地突出显示了正常背景黑素细胞，以及黑素细胞增生和恶性黑色素瘤。
结论
20分钟方案为恶性黑色素瘤免疫染色提供了一种快速可靠的方法。更快速的免疫染色方法的可用性提高了莫氏实验室的效率，并显著减少了患者和医生的等待时间。作者表示与商业支持者没有重大利益关系。
Zotero 插件