Li Chuan-jiang, Yu Li-xin, Xu Jian, Fu Shao-jie, Deng Wen-feng, Du Chuan-fu, Wang Yi-bin
Department of Organ Transplantation, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Feb;28(2):241-2.
To study the value of detection of both preoperative soluble CD30 (sCD30) and hepatocyte growth factor (HGF) level 5 days after transplantation in the diagnosis of acute rejection of renal allograft.
Preoperative serum sCD30 levels and HGF level 5 days after transplantation were determined in 65 renal-transplant recipients using enzyme-linked immunosorbent assay. The recipients were divided according to the sCD30 levels positivity. Receiver operating characteristic (ROC) curves were used to assess the value of HGF level on day 5 posttransplantation for diagnosis of acute renal allograft rejection, and the value of combined assay of the sCD30 and HGF levels was also estimated.
After transplantation, 26 recipients developed graft rejection and 39 had uneventful recovery without rejection. With the cut-off value of sCD30 of 120 U/ml, the positivity rate of sCD30 was significantly higher in recipients with graft rejection than in those without (61.5% vs 17.9%, P<0.05). Recipients with acute rejection showed also significantly higher HGF levels on day 5 posttransplantation than those without rejection (P<0.05). ROC curve analysis indicated that HGF levels on day 5 posttransplantation was a good marker for diagnosis of acute renal allograft rejection, and at the cut-off value of 90 ug/L, the diagnostic sensitivity was 84.6% and specificity 76.9%. Evaluation of both the sCD30 and HGF levels significantly enhanced the diagnostic accuracy of acute graft rejection.
Combined assay of serum sCD30 and HGF levels offers a useful means for diagnosis of acute renal allograft rejection.
研究术前可溶性CD30（sCD30）及移植后5天肝细胞生长因子（HGF）水平检测在诊断同种异体肾移植急性排斥反应中的价值。
采用酶联免疫吸附测定法测定65例肾移植受者术前血清sCD30水平及移植后5天的HGF水平。根据sCD30水平阳性情况对受者进行分组。采用受试者工作特征（ROC）曲线评估移植后第5天HGF水平对诊断同种异体肾移植急性排斥反应的价值，并评估sCD30与HGF水平联合检测的价值。
移植后，26例受者发生移植排斥反应，39例恢复顺利未发生排斥反应。以sCD30的临界值为120 U/ml时，发生移植排斥反应的受者中sCD30阳性率显著高于未发生排斥反应的受者（61.5%对17.9%，P<0.05）。发生急性排斥反应的受者在移植后第5天的HGF水平也显著高于未发生排斥反应的受者（P<0.05）。ROC曲线分析表明，移植后第5天的HGF水平是诊断同种异体肾移植急性排斥反应的良好指标，临界值为90 μg/L时，诊断敏感性为84.6%，特异性为76.9%。sCD30与HGF水平联合评估显著提高了急性移植排斥反应的诊断准确性。
血清sCD30与HGF水平联合检测为诊断同种异体肾移植急性排斥反应提供了一种有用的方法。
