构巢曲霉内切葡聚糖酶基因eglA诱导表达所需的新型启动子序列。
Novel promoter sequence required for inductive expression of the Aspergillus nidulans endoglucanase gene eglA.
作者信息
Endo Yoshikazu, Yokoyama Misako, Morimoto Munenori, Shirai Kengo, Chikamatsu Go, Kato Naoki, Tsukagoshi Norihiro, Kato Masashi, Kobayashi Tetsuo
机构信息
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya-shi, Aichi 464-8601, Japan.
出版信息
Biosci Biotechnol Biochem. 2008 Feb;72(2):312-20. doi: 10.1271/bbb.70278. Epub 2008 Feb 7.
Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Mutational analysis of the eglA promoter was performed to identify the novel cis-element responsible for XlnR-independent induction. The region spanning -153 to -138 (CCGTACCTTTTTAGGA), designated CeRE(Cellulose Responsive Element), was found to be the upstream activating element essential to inductive expression of the eglA gene.
在构巢曲霉中，编码主要内切葡聚糖酶EG A的eglA基因的表达受纤维素和纤维二糖诱导，但不受木糖诱导。这表明这种诱导独立于XlnR，XlnR是构巢曲霉中木聚糖分解和纤维素分解基因的转录激活因子。对eglA启动子进行突变分析，以鉴定负责独立于XlnR的诱导的新型顺式元件。发现跨越-153至-138（CCGTACCTTTTTAGGA）的区域，命名为CeRE（纤维素反应元件），是eglA基因诱导表达所必需的上游激活元件。
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