通过反相色谱法高效纯化小不饱和寡糖醛酸苷。

Efficient purification of small unsaturated oligoglucuronides by reversed-phase chromatography.

作者信息

Tavernier Marie-Laure, Petit Emmanuel, Lesur David, Wadouachi Anne, Courtois Bernard, Courtois Josiane, Michaud Philippe

机构信息

Laboratoire des Glucides, UMR CNRS 6219, Université de Picardie Jules Verne, 33 rue Saint-Leu, F-80039 Amiens, France.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 1;863(2):266-72. doi: 10.1016/j.jchromb.2008.01.025. Epub 2008 Jan 20.

DOI:10.1016/j.jchromb.2008.01.025

PMID:18262480

Abstract

Ion-exchange chromatography has been applied to purification of unsaturated oligoglucuronans. After an isocratic elution on a strong anion-exchange column, the collected fractions were desalted by low pressure size exclusion chromatography. However, this efficient separation was limited by the time required to desalt. So, we developed a reversed-phase chromatography method using back ionization of oligomers. Two C18 columns were tested with trifluoroacetic acid (TFA 0.7%) as eluent. Different selectivities and column stabilities were observed in this acidic condition. The scale up for semi-preparative applications enabled us to recover pure unsaturated oligoglucuronans without desalting step.

摘要

离子交换色谱法已应用于不饱和低聚葡糖醛酸聚糖的纯化。在强阴离子交换柱上进行等度洗脱后，收集的馏分通过低压尺寸排阻色谱法进行脱盐。然而，这种高效分离受到脱盐所需时间的限制。因此，我们开发了一种利用低聚物反向电离的反相色谱法。使用0.7%的三氟乙酸（TFA）作为洗脱剂对两根C18柱进行了测试。在这种酸性条件下观察到了不同的选择性和柱稳定性。半制备应用的放大使我们能够在不进行脱盐步骤的情况下回收纯的不饱和低聚葡糖醛酸聚糖。