λ作为一种克隆载体。

Lambda as a cloning vector.

作者信息

Lech K, Brent R, Irwin N

机构信息

Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.

出版信息

Curr Protoc Mol Biol. 2001 May;Chapter 1:Unit1.10. doi: 10.1002/0471142727.mb0110s00.

DOI:10.1002/0471142727.mb0110s00

PMID:18265038

Abstract

Advantages of lambda as a cloning vector are discussed along with considerations for the insert DNA (i.e., size, spi and hfl state). Maps of lambda-derived cloning vectors are provided in addition to a discussion and map of a cosmid (a lambda-derived plasmid vector).

摘要

讨论了λ作为克隆载体的优点以及对插入DNA的考虑因素（即大小、spi和hfl状态）。除了讨论粘粒（一种λ衍生的质粒载体）并给出其图谱外，还提供了λ衍生克隆载体的图谱。