粟酒裂殖酵母菌株的保存与培养基。
S. pombe strain maintenance and media.
作者信息
Forsburg Susan L
机构信息
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA.
出版信息
Curr Protoc Mol Biol. 2003 Nov;Chapter 13:Unit 13.15. doi: 10.1002/0471142727.mb1315s64.
PMID:18265319
Abstract
Fission yeast can be grown and maintained using similar culture methods to those employed for budding yeast. The optimal media for S. pombe is somewhat different than that employed for S. cerevisiae, although budding yeast media can be used if necessary. Good sterile technique is essential to prevent contamination by airborne molds or bacteria.
摘要
裂殖酵母可以使用与芽殖酵母类似的培养方法进行培养和保存。粟酒裂殖酵母的最佳培养基与酿酒酵母所用的培养基略有不同，不过如有必要，芽殖酵母的培养基也可使用。良好的无菌技术对于防止空气传播的霉菌或细菌污染至关重要。
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