Argmann Carmen A, Houten Sander M, Champy Marie-France, Auwerx Johan
Institut de Genetique et de Biologie Moleculaire et Cellulaire, Illkirch, France.
Curr Protoc Mol Biol. 2006 Aug;Chapter 29:Unit 29B.2. doi: 10.1002/0471142727.mb29b02s75.
Lipids are important body constituents that are vital for cellular, tissue, and whole-body homeostasis. Lipids serve as crucial membrane components, constitute the body's main energy reservoir, and are important signaling molecules. As a consequence of these pleiotropic functions, many common diseases, including atherosclerosis, chronic inflammatory disorders, and obesity, have been associated with altered lipid homeostasis. Lipid abnormalities are hence increasingly analyzed in mouse models. This unit describes commonly used methods to analyze mouse lipid metabolism, with techniques that evaluate lipids both in blood and in tissues. Despite the similarities between men and mice in many aspects of metabolism, important differences also exist in the area of lipid homeostasis. These differences are discussed and should be taken into account when extrapolating lipid data from mouse to men.
脂质是重要的身体组成成分，对细胞、组织及全身的稳态至关重要。脂质是关键的膜成分，构成身体的主要能量储备，并且是重要的信号分子。由于这些多效性功能，许多常见疾病，包括动脉粥样硬化、慢性炎症性疾病和肥胖症，都与脂质稳态改变有关。因此，脂质异常在小鼠模型中得到越来越多的分析。本单元描述了分析小鼠脂质代谢的常用方法，以及评估血液和组织中脂质的技术。尽管人类和小鼠在代谢的许多方面存在相似性，但在脂质稳态领域也存在重要差异。本文将讨论这些差异，并且在将小鼠脂质数据外推至人类时应予以考虑。
