Sun Taicun, Deng Zhansheng, Zhu Yong
Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu, 212001, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2007 Dec;21(12):1338-41.
To investigate the effects of insulin-like growth factor 1(IGF-1) and ethanol (EtOH) on the changes in the osteoblast proliferation and the osteoblast function under the normal serum concentration and serum starvation.
The osteoblasts harvested from the SD rat calvaria were incubated in the following six conditions according to the supplements in DMEM: the F15 group: 15% newborn calf serum (NCS); the Fis/EtOH group:100 mmol/L of EtOH added to 15% NCS; the F2 group: 2% NCS; the FZ/EtOH group: 100 mmol/L of EtOH added to 2% NCS;the F2/IGF-1 group:25 ng/ml of IGF-1 added to 2% NCS;the F2/IGF-1/EtOH group: 100 mmol/L EtOH added to 25 ng/ml IGF-1 and 2% NCS. The osteoblasts were analyzed by the MTT assay, alkaline phosphatase (ALP) activity, and RT-PCR at 24, 48, 72 and 96 hours after the culture.
The absorbance (A), the ALP activity, and the expression of BGP mRNA (the proliferation and function indicators of the osteoblasts) were significantly decreased in the F15/EtOH group at all the time points when compared with those in the F15 the group (P < 0.05); the above 3 indicators were significantly decreased in the F2 group when compared with those in the F15 group (P < 0.05); they were significantly decreased in the F2/EtOH group when compared with those in the F2 group (P < 0.05); however, the indicators in the F2/IGF-1 group were significantly increased when compared with those in the F2 group (P < 0.05); the A value in the F2/IGF-1/EtOH group was not significantly decreased when compared with that in the F2/IGF-1 group, with an exception of the A value at 24 hours (P > 0.05); however, ALP and BGP mRNA were significantly decreased (P < 0.05). All the indicators were significantly increased when compared with those in the F2/ EtOH group (P < 0.05).
Ethanol can inhibit the osteoblast proliferation and the osteoblast function, and can increase the inhibition when the osteoblasts were cultured under the serum starvation. This may be one of the mechanisms for alcoholic bone disease. IGF-1 can prevent the inhibition of the osteoblasts under the serum starvation and counteract the ethanol-induced proliferation inhibition; therefore, IGF-1 is an alternative therapeutic intervention for alcoholic bone disease.
研究胰岛素样生长因子1（IGF-1）和乙醇（EtOH）在正常血清浓度及血清饥饿条件下对成骨细胞增殖及成骨细胞功能变化的影响。
从SD大鼠颅骨获取成骨细胞，根据DMEM中的添加物在以下六种条件下进行培养：F15组：15%新生牛血清（NCS）；F15/EtOH组：在15% NCS中添加100 mmol/L EtOH；F2组：2% NCS；F2/EtOH组：在2% NCS中添加100 mmol/L EtOH；F2/IGF-1组：在2% NCS中添加25 ng/ml IGF-1；F2/IGF-1/EtOH组：在25 ng/ml IGF-1和2% NCS中添加100 mmol/L EtOH。培养24、48、72和96小时后，通过MTT法、碱性磷酸酶（ALP）活性及RT-PCR分析成骨细胞。
与F15组相比，F15/EtOH组在所有时间点的吸光度（A）、ALP活性及骨钙素（BGP）mRNA表达（成骨细胞增殖和功能指标）均显著降低（P<0.05）；与F15组相比，F2组上述3项指标显著降低（P<0.05）；与F2组相比，F2/EtOH组显著降低（P<0.05）；然而，与F2组相比，F2/IGF-1组指标显著升高（P<0.05）；与F2/IGF-1组相比，F2/IGF-1/EtOH组A值除24小时外无显著降低（P>0.05）；但ALP和BGP mRNA显著降低（P<0.05）。与F2/EtOH组相比，所有指标均显著升高（P<0.05）。
乙醇可抑制成骨细胞增殖及成骨细胞功能，且在血清饥饿条件下培养成骨细胞时可增强这种抑制作用。这可能是酒精性骨病的发病机制之一。IGF-1可防止血清饥饿条件下成骨细胞受到抑制，并抵消乙醇诱导的增殖抑制作用；因此，IGF-1是酒精性骨病的一种替代性治疗干预措施。
