He Qiang, Zhang Yi, Lu Gang, Miller Reinhard, Möhwald Helmuth, Li Junbai
Beijing National Laboratory for Molecular Sciences (BNLMS), International Joint Lab, CAS Key Lab of Colloid and Interface Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Adv Colloid Interface Sci. 2008 Aug 5;140(2):67-76. doi: 10.1016/j.cis.2007.12.004. Epub 2007 Dec 31.
Drop profile analysis tensiometry is applied to study the adsorption dynamics of phospholipids, proteins and phospholipid/protein mixtures at liquid/liquid interfaces. Measurements of the dynamic interfacial tension of phospholipid layers give information on the adsorption mechanism and the structure of the adsorption layer. The equilibrium and dynamic adsorption of pure protein solutions, i.e. human serum album (HSA), beta-lactoglobulin (beta-LG), beta-casein (beta-CA), can be explained well by the thermodynamic model of Frumkin and the diffusion-controlled adsorption theory. The adsorption behavior from mixed phospholipid/protein solutions was also investigated in terms of dynamic interfacial tensions. Interestingly, a "skin-like" folded film of pure protein or phospholipid/protein complex layers can be observed at curved surfaces at the water/oil interfaces. The addition of phospholipids accelerates the formation of the folded structure at the drop surface through co-adsorption of proteins.
滴外形分析张力测定法被用于研究磷脂、蛋白质以及磷脂/蛋白质混合物在液/液界面的吸附动力学。对磷脂层动态界面张力的测量可提供有关吸附机制和吸附层结构的信息。纯蛋白质溶液，即人血清白蛋白（HSA）、β-乳球蛋白（β-LG）、β-酪蛋白（β-CA）的平衡吸附和动态吸附，可用弗鲁姆金热力学模型和扩散控制吸附理论很好地解释。还根据动态界面张力研究了混合磷脂/蛋白质溶液的吸附行为。有趣的是，在水/油界面的弯曲表面处可以观察到纯蛋白质或磷脂/蛋白质复合层的“皮肤状”折叠膜。磷脂的添加通过蛋白质的共吸附加速了液滴表面折叠结构的形成。
