Brendel Volker
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.
Methods Mol Biol. 2007;406:521-33. doi: 10.1007/978-1-59745-535-0_25.
The accurate identification of exons and introns that comprise a complete plant gene structure can be a time-consuming and challenging task. Novel Web-based tools facilitate the process by providing a convenient interface to current transcript evidence, and portals to relevant bioinformatics software. With a few keystrokes, the user can explore alternative transcript assemblies and, for example, select for annotation those that are clearly supported by transcript evidence and similarity to known genes. The implementation of the tool at the PlantGDB resource also allows immediate communication of the novel annotations to the community through Web display.
准确识别构成完整植物基因结构的外显子和内含子可能是一项耗时且具有挑战性的任务。新型的基于网络的工具通过提供便捷的界面来获取当前转录本证据以及通向相关生物信息学软件的入口,从而推动了这一过程。只需敲击几下键盘,用户就可以探索不同的转录本组装情况,例如,选择那些有转录本证据明确支持且与已知基因相似的转录本进行注释。在植物基因数据库(PlantGDB)资源中实施该工具,还能通过网络展示将新的注释立即传达给科学界。