Ahlstrom Liisa A, Cross Sheree E, Morton John M, Mills Paul C
School of Veterinary Science, University of Queensland, Brisbane, Queensland 4072, Australia.
Vet J. 2009 Apr;180(1):48-54. doi: 10.1016/j.tvjl.2007.12.014. Epub 2008 Feb 21.
This study investigated the effects of common skin surface preparations on the penetration kinetics of hydrocortisone through canine skin. Thoracic skin from five dogs was clipped of hair, divided between five treatment groups and prepared as follows: shaved (S); tape-stripped with adhesive bandage (TS); cleaned with aqueous chlorhexidine (Aq-C); cleaned with alcoholic chlorhexidine (Al-C); or allocated to the control group and had no further preparation performed (C). The skin samples were mounted in Franz-type diffusion cells and transdermal hydrocortisone penetration was measured over 30h. The pseudo-steady-state flux (J(SS)) of hydrocortisone through S, Al-C, Aq-C and TS skin was, respectively, 2.3 (P=0.021), 2.2 (P=0.037), 2.0 (P=0.070) and 1.5 (P=0.351) times greater than through the control skin, but there were no significant differences in the lag times (t(lag)) for hydrocortisone penetration between the groups. The study has shown that some skin surface preparations can significantly increase the subsequent penetration of hydrocortisone through canine skin in vitro.
本研究调查了常见皮肤表面处理对氢化可的松经犬皮肤渗透动力学的影响。从五只犬身上采集胸部皮肤，剃去毛发，分为五个治疗组，并进行如下处理：剃毛（S）；用粘性绷带进行胶带剥离（TS）；用洗必泰水溶液清洁（Aq-C）；用洗必泰酒精溶液清洁（Al-C）；或分配到对照组，不进行进一步处理（C）。将皮肤样本安装在弗兰兹型扩散池中，在30小时内测量氢化可的松的经皮渗透。氢化可的松通过S、Al-C、Aq-C和TS皮肤的伪稳态通量（J(SS)）分别比通过对照皮肤大2.3倍（P=0.021）、2.2倍（P=0.037）、2.0倍（P=0.070）和1.5倍（P=0.351），但各组间氢化可的松渗透的滞后时间（t(lag)）无显著差异。该研究表明，一些皮肤表面处理可在体外显著增加氢化可的松随后经犬皮肤的渗透。
