Kobayashi Isao, Kuniyoshi Sawako, Saito Kazuyuki, Moritomo Tadaaki, Takahashi Tomoko, Nakanishi Teruyuki
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
Dev Comp Immunol. 2008;32(8):957-65. doi: 10.1016/j.dci.2008.01.006. Epub 2008 Feb 15.
The study of hematopoietic stem cells (HSCs) has been facilitated by the transplantation of bone marrow cell populations into lethally irradiated mice. It is widely known that HSCs have the capacity for long-term and multilineage hematopoietic reconstitution in lethally irradiated hosts. Here, we developed a transplantation model system using clonal ginbuna crucian carp (Carassius auratus langsdorfii) that were exposed to a lethal dose of X-rays. The minimum lethal dose (MLD) of ginbuna was approximately 25Gy, which is lethal due to hematopoietic failure. The transplantation of kidney hematopoietic cells into lethally irradiated ginbuna resulted in the rescue of recipient fish for more than 180 days. We examined the reconstitution activity of head kidney (HK), body kidney (BK), and spleen cells. Transplantation experiments showed that only HK and BK cells had the long-term and multilineage reconstitution activity. These results indicate that teleost HSCs have the ability to fully reconstitute the hematopoietic system in lethally irradiated hosts, and that they are present in HK and BK, but not in spleen. This transplantation model system using clonal ginbuna is useful for studies of in vivo kinetics and functions of HSCs in teleosts.
通过将骨髓细胞群体移植到接受致死剂量照射的小鼠体内，促进了对造血干细胞（HSCs）的研究。众所周知，造血干细胞在接受致死剂量照射的宿主中具有长期和多谱系造血重建的能力。在此，我们开发了一种移植模型系统，使用经致死剂量X射线照射的克隆银鲫（Carassius auratus langsdorfii）。银鲫的最小致死剂量（MLD）约为25Gy，因造血功能衰竭而致死。将肾脏造血细胞移植到接受致死剂量照射的银鲫中，可使受体鱼存活超过180天。我们检测了头肾（HK）、体肾（BK）和脾细胞的重建活性。移植实验表明，只有HK和BK细胞具有长期和多谱系重建活性。这些结果表明，硬骨鱼造血干细胞有能力在接受致死剂量照射的宿主中完全重建造血系统，并且它们存在于HK和BK中，而不存在于脾脏中。这种使用克隆银鲫的移植模型系统对于研究硬骨鱼造血干细胞的体内动力学和功能很有用。
