Zhang Suxia, Liu Zhongwei, Zhou Naiyuan, Wang Zhanhui, Shen Jianzhong
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, People's Republic of China.
Anal Chim Acta. 2008 Mar 31;612(1):83-8. doi: 10.1016/j.aca.2008.02.007. Epub 2008 Feb 9.
A homogenous complement-mediated liposome immune lysis assay (LILA) was developed for the determination of enrofloxacin (ENRO) in carp and chicken muscle. ENRO was covalently coupled to DPPE, and then immobilized onto the surface of liposomes by reverse-phase evaporation method. The performed liposome would be specifically lysed by the sequential additions of anti-ENRO monoclonal antibody (MAb) and guinea pig complement. Through a competitive assay format, the performed liposome can be used to detect ENRO in a range of 5.0-20 ng mL(-1) in assay buffer. The limit of detection of ENRO in carp and chicken muscle was 1 ng g(-1) and the limit of quantification was 2 ng g(-1). Recoveries ranged from 58.3% to 65.2% for carp and 55.6-63.8% for chicken muscle at spiked levels of 2-8 ng g(-1), with intra-assay and inter-assay variations 5.6-12.3% and 7.1-19.2%, respectively.
开发了一种均质补体介导的脂质体免疫裂解测定法（LILA），用于测定鲤鱼和鸡肉中的恩诺沙星（ENRO）。将ENRO共价偶联到二棕榈酰磷脂酰乙醇胺（DPPE）上，然后通过反相蒸发法固定在脂质体表面。通过依次添加抗ENRO单克隆抗体（MAb）和豚鼠补体，使制备的脂质体发生特异性裂解。通过竞争测定法，制备的脂质体可用于检测测定缓冲液中5.0-20 ng mL（-1）范围内的ENRO。鲤鱼和鸡肉中ENRO的检测限为1 ng g（-1），定量限为2 ng g（-1）。在2-8 ng g（-1）的加标水平下，鲤鱼的回收率为58.3%至65.2%，鸡肉的回收率为55.6-63.8%，批内和批间变异分别为5.6-12.3%和7.1-19.2%。
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