在类别转换重组过程中修复DNA断裂。
Fixing DNA breaks during class switch recombination.
作者信息
Jolly Christopher J, Cook Adam J L, Manis John P
机构信息
Centenary Institute, The University of Sydney, Sydney, NSW 2006, Australia.
出版信息
J Exp Med. 2008 Mar 17;205(3):509-13. doi: 10.1084/jem.20080356. Epub 2008 Mar 10.
Abstract
Immunoglobulin (Ig) class switch recombination (CSR) involves the breakage and subsequent repair of two DNA sequences, known as switch (S) regions, which flank IgH constant region exons. The resolution of CSR-associated breaks is thought to require the nonhomologous end-joining (NHEJ) DNA repair pathway, but the role of the NHEJ factor DNA-dependent protein kinase catalytic subunit (DNA-PKcs) in this process has been unclear. A new study, in which broken IgH-containing chromosomes in switching B cells were visualized directly, clearly demonstrated that DNA-PKcs and, unexpectedly, the nuclease Artemis are involved in the resolution of switch breaks.
摘要
免疫球蛋白（Ig）类别转换重组（CSR）涉及两个DNA序列（称为转换（S）区域）的断裂及随后的修复，这两个区域位于IgH恒定区外显子两侧。CSR相关断裂的修复被认为需要非同源末端连接（NHEJ）DNA修复途径，但NHEJ因子DNA依赖性蛋白激酶催化亚基（DNA-PKcs）在此过程中的作用尚不清楚。一项新研究直接观察了正在进行类别转换的B细胞中含有断裂IgH的染色体，清楚地表明DNA-PKcs以及出乎意料的核酸酶Artemis参与了转换断裂的修复。
相似文献
1
Fixing DNA breaks during class switch recombination.
J Exp Med. 2008 Mar 17;205(3):509-13. doi: 10.1084/jem.20080356. Epub 2008 Mar 10.
2
DNA-PKcs and Artemis function in the end-joining phase of immunoglobulin heavy chain class switch recombination.
J Exp Med. 2008 Mar 17;205(3):557-64. doi: 10.1084/jem.20080044. Epub 2008 Mar 3.
3
Artemis-independent functions of DNA-dependent protein kinase in Ig heavy chain class switch recombination and development.
Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2471-5. doi: 10.1073/pnas.0409857102. Epub 2005 Feb 7.
4
IgH class switching and translocations use a robust non-classical end-joining pathway.
Nature. 2007 Sep 27;449(7161):478-82. doi: 10.1038/nature06020. Epub 2007 Aug 22.
5
Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70.
Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):3034-9. doi: 10.1073/pnas.0915067107. Epub 2010 Jan 25.
6
DNA-PKcs Is Involved in Ig Class Switch Recombination in Human B Cells.
J Immunol. 2015 Dec 15;195(12):5608-15. doi: 10.4049/jimmunol.1501633. Epub 2015 Nov 6.
7
DNA-PKcs phosphorylation at the T2609 cluster alters the repair pathway choice during immunoglobulin class switch recombination.
Proc Natl Acad Sci U S A. 2020 Sep 15;117(37):22953-22961. doi: 10.1073/pnas.2007455117. Epub 2020 Aug 31.
8
IgH class switch recombination to IgG1 in DNA-PKcs-deficient B cells.
Immunity. 2002 Apr;16(4):607-17. doi: 10.1016/s1074-7613(02)00306-0.
9
Orientation-specific joining of AID-initiated DNA breaks promotes antibody class switching.
Nature. 2015 Sep 3;525(7567):134-139. doi: 10.1038/nature14970. Epub 2015 Aug 26.
10
The catalytic subunit of DNA-protein kinase (DNA-PKcs) is not required for Ig class-switch recombination.
Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2843-8. doi: 10.1073/pnas.0611359104. Epub 2007 Feb 12.
引用本文的文献
1
Nuclear antiviral innate responses at the intersection of DNA sensing and DNA repair.
Trends Microbiol. 2022 Nov;30(11):1056-1071. doi: 10.1016/j.tim.2022.05.004. Epub 2022 May 28.
2
IL-23 Promotes a Coordinated B Cell Germinal Center Program for Class-Switch Recombination to IgG2b in BXD2 Mice.
J Immunol. 2020 Jul 15;205(2):346-358. doi: 10.4049/jimmunol.2000280. Epub 2020 Jun 17.
3
The balancing act of R-loop biology: The good, the bad, and the ugly.
J Biol Chem. 2020 Jan 24;295(4):905-913. doi: 10.1074/jbc.REV119.011353. Epub 2019 Dec 16.
4
Purification and Biophysical Characterization of the Mre11-Rad50-Nbs1 Complex.
Methods Mol Biol. 2019;2004:269-287. doi: 10.1007/978-1-4939-9520-2_20.
5
VX-984 is a selective inhibitor of non-homologous end joining, with possible preferential activity in transformed cells.
Oncotarget. 2018 May 25;9(40):25833-25841. doi: 10.18632/oncotarget.25383.
6
Interaction of DNA repair gene polymorphisms and aflatoxin B1 in the risk of hepatocellular carcinoma.
Int J Clin Exp Pathol. 2014 Aug 15;7(9):6231-44. eCollection 2014.
7
Estimating the diversity, completeness, and cross-reactivity of the T cell repertoire.
Front Immunol. 2013 Dec 26;4:485. doi: 10.3389/fimmu.2013.00485.
8
Functional redundancy between the XLF and DNA-PKcs DNA repair factors in V(D)J recombination and nonhomologous DNA end joining.
Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):2234-9. doi: 10.1073/pnas.1222573110. Epub 2013 Jan 23.
9
Increased Artemis levels confer radioresistance to both high and low LET radiation exposures.
Radiat Oncol. 2012 Jun 19;7:96. doi: 10.1186/1748-717X-7-96.
10
B cell TLRs and induction of immunoglobulin class-switch DNA recombination.
Front Biosci (Landmark Ed). 2012 Jun 1;17(7):2594-615. doi: 10.2741/4073.
本文引用的文献
1
DNA-PKcs and Artemis function in the end-joining phase of immunoglobulin heavy chain class switch recombination.
J Exp Med. 2008 Mar 17;205(3):557-64. doi: 10.1084/jem.20080044. Epub 2008 Mar 3.
2
Non-homologous end-joining, a sticky affair.
Oncogene. 2007 Dec 10;26(56):7731-40. doi: 10.1038/sj.onc.1210871.
3
IgH class switching and translocations use a robust non-classical end-joining pathway.
Nature. 2007 Sep 27;449(7161):478-82. doi: 10.1038/nature06020. Epub 2007 Aug 22.
4
The role of activation-induced deaminase in antibody diversification and chromosome translocations.
Adv Immunol. 2007;94:75-107. doi: 10.1016/S0065-2776(06)94003-6.
5
The catalytic subunit of DNA-protein kinase (DNA-PKcs) is not required for Ig class-switch recombination.
Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2843-8. doi: 10.1073/pnas.0611359104. Epub 2007 Feb 12.
6
Pathways that suppress programmed DNA breaks from progressing to chromosomal breaks and translocations.
DNA Repair (Amst). 2006 Sep 8;5(9-10):1030-41. doi: 10.1016/j.dnarep.2006.05.024. Epub 2006 Aug 24.
7
H2AX prevents DNA breaks from progressing to chromosome breaks and translocations.
Mol Cell. 2006 Jan 20;21(2):201-14. doi: 10.1016/j.molcel.2006.01.005.
8
Role of genomic instability and p53 in AID-induced c-myc-Igh translocations.
Nature. 2006 Mar 2;440(7080):105-9. doi: 10.1038/nature04495. Epub 2006 Jan 8.
9
Artemis-independent functions of DNA-dependent protein kinase in Ig heavy chain class switch recombination and development.
Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2471-5. doi: 10.1073/pnas.0409857102. Epub 2005 Feb 7.
10
Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells.
J Exp Med. 2005 Jan 17;201(2):189-94. doi: 10.1084/jem.20040772.
Zotero 插件