在Caco-2细胞单层中，纳米铬颗粒比吡啶甲酸铬和氯化铬表现出更高的吸收效率。

Chromium nanoparticle exhibits higher absorption efficiency than chromium picolinate and chromium chloride in Caco-2 cell monolayers.

作者信息

Zha L-Y, Xu Z-R, Wang M-Q, Gu L-Y

机构信息

Key Laboratory of Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University, Hangzhou, China.

出版信息

J Anim Physiol Anim Nutr (Berl). 2008 Apr;92(2):131-40. doi: 10.1111/j.1439-0396.2007.00718.x.

DOI:10.1111/j.1439-0396.2007.00718.x

PMID:18336409

Abstract

This study was conducted to determine whether chromium nanoparticle (CrNano) exhibited higher absorption efficiency and possessed unique absorption mechanism in comparison to chromium picolinate (CrPic) and chromium chloride (CrCl(3)), as was postulated by previous reports. Twenty-one-day-old Caco-2 cell monolayers grown on semipermeable membranes in Snapwell tissue culture bichambers were incubated with CrNano, CrPic or CrCl(3) to examine their transport and uptake respectively. In the concentration range of 0.2-20 micromol/l, transport of CrNano, CrPic and CrCl(3) across Caco-2 monolayers both in apical-to-basolateral and basolateral-to-apical direction was concentration-, and time-dependent, and temperature independent. The apparent permeability coefficient (P(app)) of CrNano was between 5.89 and 7.92 x 10(-6) cm/s and that of CrPic and CrCl(3) was between 3.52 and 5.31 x 10(-6) cm/s and between 0.97 and 1.37 x 10(-6) cm/s respectively. Uptake of CrNano, CrPic and CrCl(3) by both apical and basolateral membranes was concentration- and time-dependent. Uptake of CrNano by apical membrane was significantly (p < 0.05) decreased when the incubation temperature was reduced from 37 degrees C to 4 degrees C. The transport efficiency of CrNano, CrPic and CrCl(3) after incubation for 120 min at 37 degrees C was 15.83% +/- 0.76%, 9.08% +/- 0.25% and 2.11% +/- 0.53% respectively. The uptake efficiency of CrNano, CrPic and CrCl(3) was 10.08% +/- 0.76%, 4.73% +/- 0.60% and 0.88% +/- 0.08% respectively. It was concluded that the epithelial transport of CrNano, CrPic and CrCl(3) across the Caco-2 cell monolayers was mainly via passive transport pathways. In addition, CrNano exhibited considerably higher absorption efficiency than both CrPic and CrCl(3) in Caco-2 cell monolayers.

摘要

本研究旨在确定如先前报道所假设的那样，与吡啶甲酸铬（CrPic）和氯化铬（CrCl₃）相比，纳米铬颗粒（CrNano）是否具有更高的吸收效率以及独特的吸收机制。将生长在Snapwell组织培养双室半透膜上的21日龄Caco - 2细胞单层分别与CrNano、CrPic或CrCl₃孵育，以检测它们的转运和摄取情况。在0.2 - 20微摩尔/升的浓度范围内，CrNano、CrPic和CrCl₃在顶侧到基底侧以及基底侧到顶侧方向穿过Caco - 2单层的转运呈浓度和时间依赖性，且与温度无关。CrNano的表观渗透系数（P(app)）在5.89至7.92×10⁻⁶厘米/秒之间，CrPic和CrCl₃的表观渗透系数分别在3.52至5.31×10⁻⁶厘米/秒和0.97至1.37×10⁻⁶厘米/秒之间。顶侧和基底侧膜对CrNano、CrPic和CrCl₃的摄取均呈浓度和时间依赖性。当孵育温度从37℃降至4℃时，顶侧膜对CrNano的摄取显著降低（p < 0.05）。在37℃孵育120分钟后，CrNano、CrPic和CrCl₃的转运效率分别为15.83%±0.76%、9.08%±0.25%和2.11%±0.53%。CrNano、CrPic和CrCl₃的摄取效率分别为10.08%±0.76%、4.73%±0.60%和0.88%±0.08%。研究得出结论，CrNano、CrPic和CrCl₃穿过Caco - 2细胞单层的上皮转运主要通过被动转运途径。此外，在Caco - 2细胞单层中，CrNano的吸收效率明显高于CrPic和CrCl₃。