Effects of potassium depolarization on intracellular compartmentalization of ATP in cholinergic synaptosomes isolated from Torpedo electric organ.

It is well known that acetylcholine (ACh) and ATP are co-stored and co-released in nerve terminals of the electric organ of Torpedo. Cholinergic synaptosomes were subjected to a cycle of freezing and thawing showing that ATP is distributed in two operational pools like those described for ACh. The bound pool is resistant to freezing and thawing, and it is presumably protected by membranes. When metabolically active ATP was prelabelled with [3H]adenosine, 76% of the radioactivity was associated with the free pool of ATP. When the preparation was depolarized in a calcium containing medium, there was a decrease in the specific radioactivity of ATP in the free pool and an increase in the bound pool. These results reflect that the patterns of distribution of ACh and ATP, in this synaptosomal preparation, are similar in resting conditions and during K+ depolarization.