The effect of pentoxifylline on oxidative stress in CO2 pneumoperitoneum.

BACKGROUND

Carbon dioxide (CO(2)) pneumoperitoneum induces peritoneal oxidative stress. This experimental, randomized, controlled study was designed to investigate the effect of pentoxifylline on oxidative stress induced by CO(2 )pneumoperitoneum.

METHODS

For this study, 36 Swiss albino rats were randomized into three groups. Arteria, vena femoralis, and peritoneal cavity were cannulated after anesthesia. The arterial pH, partial arterial oxygen pressure (PaO(2)), venous PO(2), arterial and venous PO(2) difference (P((a-v))O(2)), serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), and thiobarbituric acid-reactive substances (TBARS) were studied at the end of the first and second hours in group 1 (control). In group 2, 1 cc isotonic NaCl was injected into peritoneal cavity and then CO(2) pneumoperitoneum was established. At the end of the first hour of insufflation and one hour after desufflation, the same parameters as in group 1 were studied. In group 3, the CO(2) pneumoperitoneum plus pentoxifylline group, all procedures as in group 2 were repeated, with the exception of pentoxifylline (50 mg/kg) injected in place of saline.

RESULTS

At the end of the first hour, P((a-v))O(2 )value in group 2 was significantly less than in the control group (group 1) and group 3 (p \ 0.05). There were no significant differences in PaO(2, )pH, AST, and ALT values between groups (p [ 0.05). TBARS level in group 1 was significantly lower than in the other groups, but there was no significant difference in TBARS level between groups 2 and 3. At the end of the second hour, TBARS level in group 3 was significantly lower than in group 2 (p \ 0.05).

CONCLUSIONS

Pentoxifylline may reduce the oxidative injury following laparoscopic procedures.