嘌呤能P2受体在实验性视网膜新生血管形成中的作用。

Involvement of purinergic P2 receptors in experimental retinal neovascularization.

作者信息

Sarman Sylvia, Mancini Jorge, van der Ploeg Ingeborg, Croxatto J Oscar, Kvanta Anders, Gallo Juan E

机构信息

Department of Clinical Neuroscience, St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden.

出版信息

Curr Eye Res. 2008 Mar;33(3):285-91. doi: 10.1080/02713680701885470.

DOI:10.1080/02713680701885470

PMID:18350440

Abstract

PURPOSE

The present study was aimed to investigate the expression of purinergic P2 receptors in oxygen-induced retinal neovascularization.

METHODS

Immunohistochemistry was used to study the expression of purinergic P2Y2 and P2X2 receptors in the neonatal mouse retina during normal vascular development and after oxygen-induced retinopathy (OIR). The effect of the P2 antagonists, suramin and PPADS, on the extent of oxygen-induced retinal neovascularization was analyzed.

RESULTS

In normal mice, the expression of P2Y2 receptors was weak throughout the retina, whereas P2X2 receptor expression was detected in the outer plexiform layer. In mice treated with oxygen, P2Y2 expression was detected in the ganglion and in the nerve fiber layers, whereas P2X2 expression was found in the inner and outer plexiform layers. Oxygen-induced preretinal neovascularization was strongly inhibited by the P2 antagonists, suramin (p<0.05) and PPADS (p<0.05), and this was accompanied by a down-regulation of P2X2 receptor expression in the inner plexiform layer in suramin-treated mice.

CONCLUSIONS

The data suggest that purinergic P2 receptors are involved in neovascularization associated with OIR.

摘要

目的

本研究旨在探讨嘌呤能P2受体在氧诱导性视网膜新生血管形成中的表达。

方法

采用免疫组织化学方法研究嘌呤能P2Y2和P2X2受体在新生小鼠视网膜正常血管发育过程及氧诱导性视网膜病变（OIR）后的表达情况。分析P2拮抗剂苏拉明和吡哆醛-6-偶氮-2',4'-二磺酸（PPADS）对氧诱导性视网膜新生血管形成程度的影响。

结果

在正常小鼠中，P2Y2受体在整个视网膜中的表达较弱，而P2X2受体表达则在外丛状层被检测到。在用氧处理的小鼠中，P2Y2表达在神经节和神经纤维层被检测到，而P2X2表达在内、外丛状层被发现。氧诱导的视网膜前新生血管形成受到P2拮抗剂苏拉明（p<0.05）和PPADS（p<0.05）的强烈抑制，并且在苏拉明处理的小鼠中，这伴随着内丛状层中P2X2受体表达的下调。