OBJECTIVE

To investigate the effects on forming of hypertrophic scar after BMSCs infected with adenovirus carrying TGF-beta3c2s2 were transplanted into the wound of animal scar model.

METHODS

The third passage of rabbit's BMSCs were infected with 150 mutiple infection, and were cultured 24 hours. The concentration of the BMSCs infected with recombinant adenovirus containing the TGF-beta3c2s2 gene was 1 x 10(5) cell/mL. The purified and evaporated recombinant adenovirus grains containing the TGF-beta3c2s2 gene were diluted by DMEM/F12 (without FBS) to 1 x 10(8) pfu/mL. The animal scar model of the standard Japanese big ear rabbit was established. Eighty wounds were generated on the gastroside of ear and were randomized to 4 groups in each rabbit, which were divided into 3 control groups (A: control, B: Ad-TGF-beta3c2s2, C: BMSCs) and 1 experimental group (D: BMSCs/Ad-TGF-beta3c2s2). Then the wounds were tranplanted with cells. On 45 days and 90 days after wounded, thickness and hardness of scars were measured with color ultrasound diagnostic unit and especial measurement for skin and scar hardness. On 21, 45 and 90 days, three specimens were harvested respectively for further histological study.

RESULTS

The wound of groups A, B, C gradually formed the different degree scars after epithelialization. The hyperplasty of scars reached peak on 45 days after wounded and lasted about 90 days. There was no prominent scar formed in group D during the whole observed procedure. Thickness and hardness of scar of group D and group E were approximate on 45 days and 90 days. Thickness and hardness of scar of groups A, B and C were lower than those of group D (P < 0.01), and group B showed more lower than group A and group C (P < 0.01). Disorder structure and overlapping arrangement, enlargement collagen fibers were showed in the HE histological sections of the scars of groups A, C. The structure of the scars of groups B, C were similar to Group E. The constitutions of groups A, B, C, D on 90 days resembled to each one on 45 days. In section of immunohistochemistry after wounded on 21 days and 45 days, positive expressions of BrdU in nucleus of Groups C, D were observed. Negative expressions of BrdU in Groups A, B, E were showed.

CONCLUSION

BMSCs with Ad-TGFbeta3c2s2 gene transplanted into wound could inhibit the forming of hypertrophic scar.