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具有增强灵敏度的双指纹试剂:5-甲氧基茚三酮和5-甲硫基茚三酮

Dual fingerprint reagents with enhanced sensitivity: 5-methoxy- and 5-methylthioninhydrin.

作者信息

Almog Joseph, Klein Asne, Davidi Inbal, Cohen Yaron, Azoury Myriam, Levin-Elad Michal

机构信息

Casali Institute of Applied Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel.

出版信息

J Forensic Sci. 2008 Mar;53(2):364-8. doi: 10.1111/j.1556-4029.2008.00671.x.

Abstract

"Dual fingerprint reagents" are chemical formulations which produce with latent fingerprints in a single step, impressions that are both colored and fluorescent. Pre-mixed solutions of the two commercially available ninhydrin analogues, 5-methoxyninhydrin (MN) and 5-methylthioninhydrin (MTN) with zinc or cadmium salts, are true dual reagents. They are much more sensitive than the parent dual reagent, ninhydrin/ZnCl(2). The main advantage of the new formulations is that they can be used at room temperature, with no need to cool the sample to liquid nitrogen temperature. At 0.05% concentration, which is 10-fold lower than the common ninhydrin working solution, MTN/ZnCl(2) is as sensitive as DFO in the fluorescence mode and considerably more sensitive in the color mode. MTN is also slightly cheaper than DFO.

摘要

“双指纹试剂”是一类化学制剂,可一步产生带潜指纹的有色且荧光的印记。两种市售茚三酮类似物5-甲氧基茚三酮(MN)和5-甲基硫代茚三酮(MTN)与锌盐或镉盐的预混溶液是真正的双试剂。它们比母体双试剂茚三酮/ZnCl₂灵敏得多。新制剂的主要优点是可在室温下使用,无需将样品冷却至液氮温度。在浓度为0.05%(比常见茚三酮工作溶液低10倍)时,MTN/ZnCl₂在荧光模式下与DFO一样灵敏,在颜色模式下则灵敏得多。MTN也比DFO略便宜。

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