Silva-García R, Estrada-García I, Ramos-Payán R, Torres-Salazar A, Morales-Martínez M E, Arenas-Aranda D, Giménez-Scherer J A, Blanco-Favela F, Rico-Rosillo M G
Centro Médico Nacional Siglo XXI, Unidad de Investigación Médica en Inmunología, Instituto Mexicano del Seguro Social, Hospital de Pediatría, Avenida Cuauhtémoc 330, Col. Doctores, C P 06720, México, D F, México.
Inflamm Res. 2008 Apr;57(4):145-50. doi: 10.1007/s00011-007-6199-y.
Evaluate the Monocyte Locomotion Inhibitory Factor (MLIF) effect upon the expression of genes encoding human cytokines, receptors and related factors in the human cell line U-937. MLIF (Met-Gln-Cys-Asn-Ser) is an anti-inflammatory pentapeptide produced by Entamoeba histolytica that inhibits many human monocyte functions.
U-937 cell line cultured (24 hrs/RPMI). RNA extracted by Trizol method. 385 genes were analyzed on microarray membranes, complement by real-time RT-PCR and protein expression of some affected genes.
MLIF had a preferentially inhibitory effect on gene expression; four genes were over-expressed and 13 underexpressed in MILF vs. simple medium - constitutive expression. Three genes are over-expressed and 19 under-expressed in MLIF/PMA vs. PMA - induced expression.
Many modified genes are products regulated by the Nuclear Factor-kappaB and Mitogen Activated Protein Kinase pathways, suggesting MLIF involvement with these two major pathways for the modulation of the inflammation and immune responses.
评估单核细胞移动抑制因子（MLIF）对人细胞系U - 937中编码人类细胞因子、受体及相关因子的基因表达的影响。MLIF（甲硫氨酸 - 谷氨酰胺 - 半胱氨酸 - 天冬酰胺 - 丝氨酸）是一种由溶组织内阿米巴产生的抗炎五肽，可抑制多种人类单核细胞功能。
培养U - 937细胞系（24小时/ RPMI培养基）。采用Trizol法提取RNA。在微阵列膜上分析385个基因，并通过实时逆转录 - 聚合酶链反应及部分受影响基因的蛋白质表达进行补充。
与单纯培养基组成型表达相比，MLIF对基因表达具有优先抑制作用；在MLIF处理组中，4个基因过度表达，13个基因表达不足。与PMA诱导表达相比，在MLIF/PMA处理组中，3个基因过度表达，19个基因表达不足。
许多被改变的基因是由核因子 - κB和丝裂原活化蛋白激酶途径调控的产物，这表明MLIF参与了这两条调节炎症和免疫反应的主要途径。
