Liu Nai-You, Zhang Zhi-Feng, Yang Wei-Cai
The Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, China.
Methods Mol Biol. 2008;427:91-100. doi: 10.1007/978-1-59745-273-1_7.
As a model system for efficient analysis of plant gene function, Arabidopsis can easily be transformed by Agrobacterium-mediated gene transfer. Among the various transformation methods developed during the past two decades, the vacuum infiltration transformation and the floral dip method are most widely used. Compared with conventional plant transformation methods that involve tissue culture and plant regeneration, vacuum infiltration and the floral dip method require minimal time and labor; furthermore, these two high-throughput transformation procedures can also be used in insertional mutagenesis, one of the most powerful tools for studying gene function. Here, we outline a detailed description with various fine-tuned steps for these two Agrobacterium-mediated floral transformation methods in Arabidopsis.
作为一种用于高效分析植物基因功能的模式系统，拟南芥可以通过农杆菌介导的基因转移轻松实现转化。在过去二十年中开发的各种转化方法中，真空渗透转化法和花浸染法应用最为广泛。与涉及组织培养和植株再生的传统植物转化方法相比，真空渗透法和花浸染法所需的时间和人力最少；此外，这两种高通量转化方法还可用于插入诱变，这是研究基因功能最强大的工具之一。在此，我们概述了拟南芥中这两种农杆菌介导的花转化方法的详细描述及各种微调步骤。
