利用三（联吡啶）钌（II）过二硫酸盐体系，醛衍生化后青霉胺和麻黄碱化学发光增强及其分析应用。

Enhancement of the chemiluminescence of penicillamine and ephedrine after derivatization with aldehydes using tris(bipyridyl)ruthenium(II) peroxydisulfate system and its analytical application.

作者信息

Suliman Fakhr Eldin O, Al-Hinai Mohammed M, Al-Kindy Salma M Z, Salama Salama B

机构信息

Department of Chemistry, College of Science, Sultan Qaboos University, Box 36, Al-Khod 123, Oman.

出版信息

Talanta. 2008 Feb 15;74(5):1256-64. doi: 10.1016/j.talanta.2007.08.040. Epub 2007 Sep 7.

DOI:10.1016/j.talanta.2007.08.040

PMID:18371778

Abstract

A novel sequential injection (SI) method was developed for the determination of penicillamine (PA) and ephedrine (EP) based on the reaction of these drugs with tris(bipyridyl)ruthenium(II) (Ru(bpy)(3)(2+)) and peroxydisulfate (S(2)O(8)(2-)) in the presence of light. Derivatization of PA and EP with aldehydes has resulted in a significant enhancement of the chemiluminescence emission signal by at least 25 times for PA and 12 times for EP, leading to better sensitivities and lower detection limits for both drugs. The instrumental setup utilized a syringe pump and a multiposition valve to aspirate the reagents, (Ru(bpy)(3)(2+) and S(2)O(8)(2-)), and a peristaltic pump to propel the sample. The experimental conditions affecting the derivatization reaction and the chemiluminescence reaction were systematically optimized using the univariate approach. Under the optimum conditions linear calibration curves between 0.2-24 microgmL(-1) for PA and 0.2-20 microgmL(-1) for EP were obtained. The detection limits were 0.1 microgmL(-1) for PA and 0.03 microgmL(-1) for EP. The procedure was applied to the analysis of PA and EP in pharmaceutical products and was found to be free from interferences from concomitants usually present in these preparations.

摘要

基于青霉胺（PA）和麻黄碱（EP）在光照条件下与三（联吡啶）钌（II）（Ru(bpy)(3)(2+)）和过二硫酸盐（S(2)O(8)(2-)）的反应，开发了一种用于测定PA和EP的新型顺序注射（SI）方法。PA和EP与醛的衍生化反应使化学发光发射信号显著增强，PA至少增强25倍，EP增强12倍，从而提高了两种药物的灵敏度并降低了检测限。仪器装置使用注射泵和多位置阀吸取试剂（Ru(bpy)(3)(2+)和S(2)O(8)(2-)），并使用蠕动泵推动样品。采用单变量方法系统地优化了影响衍生化反应和化学发光反应的实验条件。在最佳条件下，获得了PA在0.2 - 24 μgmL(-1)和EP在0.2 - 20 μgmL(-1)之间的线性校准曲线。PA的检测限为0.1 μgmL(-1)，EP的检测限为0.03 μgmL(-1)。该方法应用于药品中PA和EP的分析，发现不受这些制剂中通常存在的共存物的干扰。